Results 41 - 49 of 49 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
|
R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 15848 | 5' | -56.3 | NC_004065.1 | + | 184901 | 0.68 | 0.852941 |
|
Target: 5'- aUCGGGAGCGggcGCCCGG-GUgGCUCGg -3' miRNA: 3'- aGGUCCUCGU---CGGGCUaUAgCGAGUg -5' |
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| 15848 | 5' | -56.3 | NC_004065.1 | + | 189085 | 0.66 | 0.939659 |
|
Target: 5'- -gCGGGGGCucgaggaccucGCCCGGUGUCucuguGCUCAg -3' miRNA: 3'- agGUCCUCGu----------CGGGCUAUAG-----CGAGUg -5' |
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| 15848 | 5' | -56.3 | NC_004065.1 | + | 195103 | 0.72 | 0.659869 |
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Target: 5'- cCCAGGGGCGucagcucGCCggCGGUGUCGC-CGCa -3' miRNA: 3'- aGGUCCUCGU-------CGG--GCUAUAGCGaGUG- -5' |
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| 15848 | 5' | -56.3 | NC_004065.1 | + | 196648 | 0.66 | 0.934927 |
|
Target: 5'- aUCCAGGGGCcaguAGCgCgCGGcGUCuCUCACg -3' miRNA: 3'- -AGGUCCUCG----UCG-G-GCUaUAGcGAGUG- -5' |
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| 15848 | 5' | -56.3 | NC_004065.1 | + | 196713 | 0.68 | 0.852941 |
|
Target: 5'- aUCCGGGGGUguacAGCgCGAcGUCGCgccCGCu -3' miRNA: 3'- -AGGUCCUCG----UCGgGCUaUAGCGa--GUG- -5' |
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| 15848 | 5' | -56.3 | NC_004065.1 | + | 203391 | 0.68 | 0.875106 |
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Target: 5'- uUCCGGGAcGCugcuCCCGcUGUCGC-CGCa -3' miRNA: 3'- -AGGUCCU-CGuc--GGGCuAUAGCGaGUG- -5' |
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| 15848 | 5' | -56.3 | NC_004065.1 | + | 209147 | 0.68 | 0.888848 |
|
Target: 5'- cCCAGcgccGCGGCC--GUGUCGCUCGCc -3' miRNA: 3'- aGGUCcu--CGUCGGgcUAUAGCGAGUG- -5' |
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| 15848 | 5' | -56.3 | NC_004065.1 | + | 211583 | 0.68 | 0.888848 |
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Target: 5'- aUCCGGcGGCuGUCCGAggucUCGCUCGu -3' miRNA: 3'- -AGGUCcUCGuCGGGCUau--AGCGAGUg -5' |
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| 15848 | 5' | -56.3 | NC_004065.1 | + | 227266 | 0.66 | 0.929965 |
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Target: 5'- gUCCAGaGAcaCAGCCUuaguacacauauGGUAUCGUUCACc -3' miRNA: 3'- -AGGUC-CUc-GUCGGG------------CUAUAGCGAGUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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