Results 41 - 49 of 49 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 15848 | 5' | -56.3 | NC_004065.1 | + | 43096 | 0.66 | 0.944164 |
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Target: 5'- gUCCGGGcauccGCgacccGGCgCCGAUccgCGCUCGCg -3' miRNA: 3'- -AGGUCCu----CG-----UCG-GGCUAua-GCGAGUG- -5' |
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| 15848 | 5' | -56.3 | NC_004065.1 | + | 150178 | 0.66 | 0.948444 |
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Target: 5'- cUUguGGAugcGCAGCUCGuuGUGUCGUUUGCg -3' miRNA: 3'- -AGguCCU---CGUCGGGC--UAUAGCGAGUG- -5' |
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| 15848 | 5' | -56.3 | NC_004065.1 | + | 169126 | 0.66 | 0.944164 |
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Target: 5'- gCCGcGGccAGCAGuuCCCGAga-CGCUCGCg -3' miRNA: 3'- aGGU-CC--UCGUC--GGGCUauaGCGAGUG- -5' |
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| 15848 | 5' | -56.3 | NC_004065.1 | + | 148796 | 0.66 | 0.934927 |
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Target: 5'- cCCAGGAacgacgaccgGCAGUCCGAguccgCGCgguacugCGCg -3' miRNA: 3'- aGGUCCU----------CGUCGGGCUaua--GCGa------GUG- -5' |
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| 15848 | 5' | -56.3 | NC_004065.1 | + | 196648 | 0.66 | 0.934927 |
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Target: 5'- aUCCAGGGGCcaguAGCgCgCGGcGUCuCUCACg -3' miRNA: 3'- -AGGUCCUCG----UCG-G-GCUaUAGcGAGUG- -5' |
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| 15848 | 5' | -56.3 | NC_004065.1 | + | 141002 | 0.67 | 0.907826 |
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Target: 5'- aCCGGcAGCuGCCUGAgacacaaccucgUGUCGUUCGCc -3' miRNA: 3'- aGGUCcUCGuCGGGCU------------AUAGCGAGUG- -5' |
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| 15848 | 5' | -56.3 | NC_004065.1 | + | 127214 | 0.67 | 0.901723 |
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Target: 5'- gUUCGGGAGCggcGGCCgCGAggAUCGggCGCg -3' miRNA: 3'- -AGGUCCUCG---UCGG-GCUa-UAGCgaGUG- -5' |
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| 15848 | 5' | -56.3 | NC_004065.1 | + | 184901 | 0.68 | 0.852941 |
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Target: 5'- aUCGGGAGCGggcGCCCGG-GUgGCUCGg -3' miRNA: 3'- aGGUCCUCGU---CGGGCUaUAgCGAGUg -5' |
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| 15848 | 5' | -56.3 | NC_004065.1 | + | 137999 | 0.78 | 0.371525 |
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Target: 5'- cUCCGGGuGUacGGUCUGAUAUCGCUUGCg -3' miRNA: 3'- -AGGUCCuCG--UCGGGCUAUAGCGAGUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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