Results 21 - 30 of 30 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 15852 | 3' | -56.5 | NC_004065.1 | + | 151380 | 0.67 | 0.915822 |
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Target: 5'- uGCGCUgGCGgccGAUcucuUGGGUGGCGGAg -3' miRNA: 3'- -UGCGGgUGUau-CUAc---ACCCGCUGCCU- -5' |
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| 15852 | 3' | -56.5 | NC_004065.1 | + | 164343 | 0.75 | 0.501409 |
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Target: 5'- cACGCCCGCGc-GAUGUccucGGGCGuCGGAu -3' miRNA: 3'- -UGCGGGUGUauCUACA----CCCGCuGCCU- -5' |
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| 15852 | 3' | -56.5 | NC_004065.1 | + | 165232 | 0.66 | 0.953639 |
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Target: 5'- cACGCUCAC--GGAUG-GGGUcagGAUGGGg -3' miRNA: 3'- -UGCGGGUGuaUCUACaCCCG---CUGCCU- -5' |
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| 15852 | 3' | -56.5 | NC_004065.1 | + | 167585 | 0.66 | 0.945518 |
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Target: 5'- uUGUCgACGUcuGAUGUGGGUgcuGGCGGGg -3' miRNA: 3'- uGCGGgUGUAu-CUACACCCG---CUGCCU- -5' |
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| 15852 | 3' | -56.5 | NC_004065.1 | + | 197811 | 0.66 | 0.941129 |
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Target: 5'- aGCGCCCGCGgcgccacguugGGGUcGUccaggucccagGGGCGGCaGGAg -3' miRNA: 3'- -UGCGGGUGUa----------UCUA-CA-----------CCCGCUG-CCU- -5' |
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| 15852 | 3' | -56.5 | NC_004065.1 | + | 200540 | 0.69 | 0.833054 |
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Target: 5'- gGCGUCCAUGgcGA---GGGCGACGGGc -3' miRNA: 3'- -UGCGGGUGUauCUacaCCCGCUGCCU- -5' |
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| 15852 | 3' | -56.5 | NC_004065.1 | + | 200806 | 0.67 | 0.897952 |
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Target: 5'- gGCGUCUcggcGCGgGGAUcGgacgGGGCGACGGGg -3' miRNA: 3'- -UGCGGG----UGUaUCUA-Ca---CCCGCUGCCU- -5' |
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| 15852 | 3' | -56.5 | NC_004065.1 | + | 201405 | 0.66 | 0.953639 |
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Target: 5'- -aGUUgGCGUguguugGGAUGUGGGuCGugGGGa -3' miRNA: 3'- ugCGGgUGUA------UCUACACCC-GCugCCU- -5' |
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| 15852 | 3' | -56.5 | NC_004065.1 | + | 212340 | 0.69 | 0.85645 |
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Target: 5'- gACGgCCACGgcGAUG-GcGCGACGGAc -3' miRNA: 3'- -UGCgGGUGUauCUACaCcCGCUGCCU- -5' |
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| 15852 | 3' | -56.5 | NC_004065.1 | + | 223132 | 0.66 | 0.936516 |
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Target: 5'- cCG-CCGCGUAGAUGaGGGCGAgcauccCGGc -3' miRNA: 3'- uGCgGGUGUAUCUACaCCCGCU------GCCu -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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