Results 21 - 30 of 30 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 15852 | 3' | -56.5 | NC_004065.1 | + | 33796 | 0.66 | 0.936516 |
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Target: 5'- -gGCCCgACAUgcGGAcGUGGGaCGACGcGGa -3' miRNA: 3'- ugCGGG-UGUA--UCUaCACCC-GCUGC-CU- -5' |
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| 15852 | 3' | -56.5 | NC_004065.1 | + | 151380 | 0.67 | 0.915822 |
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Target: 5'- uGCGCUgGCGgccGAUcucuUGGGUGGCGGAg -3' miRNA: 3'- -UGCGGgUGUau-CUAc---ACCCGCUGCCU- -5' |
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| 15852 | 3' | -56.5 | NC_004065.1 | + | 103694 | 0.67 | 0.910087 |
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Target: 5'- -aGaCCUACAcg---GUGGGCGGCGGGg -3' miRNA: 3'- ugC-GGGUGUaucuaCACCCGCUGCCU- -5' |
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| 15852 | 3' | -56.5 | NC_004065.1 | + | 112 | 0.67 | 0.909501 |
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Target: 5'- cCGCCCaugcaagugaaugGCGUGGA-GUGGGUGuuGGGc -3' miRNA: 3'- uGCGGG-------------UGUAUCUaCACCCGCugCCU- -5' |
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| 15852 | 3' | -56.5 | NC_004065.1 | + | 129981 | 0.67 | 0.897952 |
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Target: 5'- gUGUCCGCAgaacgccGcgG-GGGCGGCGGAc -3' miRNA: 3'- uGCGGGUGUau-----CuaCaCCCGCUGCCU- -5' |
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| 15852 | 3' | -56.5 | NC_004065.1 | + | 212340 | 0.69 | 0.85645 |
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Target: 5'- gACGgCCACGgcGAUG-GcGCGACGGAc -3' miRNA: 3'- -UGCgGGUGUauCUACaCcCGCUGCCU- -5' |
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| 15852 | 3' | -56.5 | NC_004065.1 | + | 309 | 0.71 | 0.726049 |
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Target: 5'- cGCGCCCGCG-AGAcGUGuacGGCGugGGu -3' miRNA: 3'- -UGCGGGUGUaUCUaCAC---CCGCugCCu -5' |
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| 15852 | 3' | -56.5 | NC_004065.1 | + | 164343 | 0.75 | 0.501409 |
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Target: 5'- cACGCCCGCGc-GAUGUccucGGGCGuCGGAu -3' miRNA: 3'- -UGCGGGUGUauCUACA----CCCGCuGCCU- -5' |
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| 15852 | 3' | -56.5 | NC_004065.1 | + | 83284 | 1.09 | 0.004228 |
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Target: 5'- gACGCCCACAUAGAUGUGGGCGACGGAg -3' miRNA: 3'- -UGCGGGUGUAUCUACACCCGCUGCCU- -5' |
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| 15852 | 3' | -56.5 | NC_004065.1 | + | 201405 | 0.66 | 0.953639 |
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Target: 5'- -aGUUgGCGUguguugGGAUGUGGGuCGugGGGa -3' miRNA: 3'- ugCGGgUGUA------UCUACACCC-GCugCCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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