Results 61 - 68 of 68 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 15857 | 3' | -54.7 | NC_004065.1 | + | 197120 | 0.66 | 0.979247 |
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Target: 5'- uCGUCGccuccgacccccgacAGGACGGCAUCUCGgACg-- -3' miRNA: 3'- cGCGGC---------------UCCUGUCGUAGAGCaUGagc -5' |
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| 15857 | 3' | -54.7 | NC_004065.1 | + | 165745 | 0.66 | 0.979679 |
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Target: 5'- cGCGCCGcGccuuccaggaacucGGACAGCGUCU-GUagcacccGCUCGu -3' miRNA: 3'- -CGCGGC-U--------------CCUGUCGUAGAgCA-------UGAGC- -5' |
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| 15857 | 3' | -54.7 | NC_004065.1 | + | 86364 | 0.66 | 0.980315 |
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Target: 5'- -aGCCGAGGcucuCGGCcacgucgggguagGUCUCGgcCUCa -3' miRNA: 3'- cgCGGCUCCu---GUCG-------------UAGAGCauGAGc -5' |
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| 15857 | 3' | -54.7 | NC_004065.1 | + | 183506 | 0.66 | 0.980524 |
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Target: 5'- gGUGCaCGAccGACAGgGUCUCGaacgGCUCGc -3' miRNA: 3'- -CGCG-GCUc-CUGUCgUAGAGCa---UGAGC- -5' |
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| 15857 | 3' | -54.7 | NC_004065.1 | + | 32790 | 0.66 | 0.980524 |
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Target: 5'- uGCGCuCGAGGGC-----CUCGUugUCGu -3' miRNA: 3'- -CGCG-GCUCCUGucguaGAGCAugAGC- -5' |
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| 15857 | 3' | -54.7 | NC_004065.1 | + | 24922 | 0.66 | 0.980524 |
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Target: 5'- gGCGCCauaucGAGcgaucGACAGCGUUUuCGaGCUCGa -3' miRNA: 3'- -CGCGG-----CUC-----CUGUCGUAGA-GCaUGAGC- -5' |
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| 15857 | 3' | -54.7 | NC_004065.1 | + | 109196 | 0.66 | 0.98252 |
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Target: 5'- -gGCCGGcGGCGGCGcCUCGgccucuuccUGCUCGg -3' miRNA: 3'- cgCGGCUcCUGUCGUaGAGC---------AUGAGC- -5' |
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| 15857 | 3' | -54.7 | NC_004065.1 | + | 179440 | 0.66 | 0.98252 |
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Target: 5'- gGCGcCCGAaGACAGUAgg--GUGCUCGa -3' miRNA: 3'- -CGC-GGCUcCUGUCGUagagCAUGAGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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