Results 61 - 68 of 68 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 15857 | 3' | -54.7 | NC_004065.1 | + | 189088 | 0.7 | 0.878353 |
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Target: 5'- gGgGCuCGAGGACcucgcccGGUGUCUCuGUGCUCa -3' miRNA: 3'- -CgCG-GCUCCUG-------UCGUAGAG-CAUGAGc -5' |
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| 15857 | 3' | -54.7 | NC_004065.1 | + | 196628 | 0.7 | 0.885862 |
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Target: 5'- uGCGCCG-GGGCgAGCGcCUCGagagGCUCc -3' miRNA: 3'- -CGCGGCuCCUG-UCGUaGAGCa---UGAGc -5' |
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| 15857 | 3' | -54.7 | NC_004065.1 | + | 199921 | 0.69 | 0.905019 |
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Target: 5'- cCGCCGGGGcuGCAGC-UCUCGcUAC-CGc -3' miRNA: 3'- cGCGGCUCC--UGUCGuAGAGC-AUGaGC- -5' |
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| 15857 | 3' | -54.7 | NC_004065.1 | + | 186553 | 0.68 | 0.941899 |
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Target: 5'- gGCGCCGAGGACA------CGUGCUgCGg -3' miRNA: 3'- -CGCGGCUCCUGUcguagaGCAUGA-GC- -5' |
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| 15857 | 3' | -54.7 | NC_004065.1 | + | 130597 | 0.68 | 0.941899 |
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Target: 5'- aUGUCGAGG-CGGCGUCUgccGCUCGg -3' miRNA: 3'- cGCGGCUCCuGUCGUAGAgcaUGAGC- -5' |
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| 15857 | 3' | -54.7 | NC_004065.1 | + | 144078 | 0.68 | 0.941899 |
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Target: 5'- cGCGUCGAGGACGGCGaugagaUCGgcgACg-- -3' miRNA: 3'- -CGCGGCUCCUGUCGUag----AGCa--UGagc -5' |
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| 15857 | 3' | -54.7 | NC_004065.1 | + | 59331 | 0.68 | 0.944979 |
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Target: 5'- cGCGCCGcGGGCGGCGUgcgauuuccagcucCgagugCGUGgUCGa -3' miRNA: 3'- -CGCGGCuCCUGUCGUA--------------Ga----GCAUgAGC- -5' |
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| 15857 | 3' | -54.7 | NC_004065.1 | + | 80559 | 1.11 | 0.004752 |
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Target: 5'- gGCGCCGAGGACAGCAUCUCGUACUCGa -3' miRNA: 3'- -CGCGGCUCCUGUCGUAGAGCAUGAGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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