Results 21 - 25 of 25 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
1586 | 3' | -52.2 | NC_001347.2 | + | 95471 | 0.66 | 0.991812 |
Target: 5'- -gGCGC-GCGUCAcgcGGCgccGGUGuCGGCg -3' miRNA: 3'- uaCGUGaCGCAGU---UUGa--CCACuGCUG- -5' |
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1586 | 3' | -52.2 | NC_001347.2 | + | 79316 | 0.66 | 0.992864 |
Target: 5'- gGUGCGCgGCGaCAAGCU-GUGGCuGCa -3' miRNA: 3'- -UACGUGaCGCaGUUUGAcCACUGcUG- -5' |
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1586 | 3' | -52.2 | NC_001347.2 | + | 88680 | 0.66 | 0.992864 |
Target: 5'- -gGCGCcgGCG-CGcccuGCUGG-GACGACg -3' miRNA: 3'- uaCGUGa-CGCaGUu---UGACCaCUGCUG- -5' |
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1586 | 3' | -52.2 | NC_001347.2 | + | 125029 | 0.66 | 0.993806 |
Target: 5'- -gGCgGCUGCuGUugcaCGGGCUcgGGUGACGGCg -3' miRNA: 3'- uaCG-UGACG-CA----GUUUGA--CCACUGCUG- -5' |
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1586 | 3' | -52.2 | NC_001347.2 | + | 1849 | 0.66 | 0.993806 |
Target: 5'- -cGCACcccGCGUC--GCUGcUGACGGCc -3' miRNA: 3'- uaCGUGa--CGCAGuuUGACcACUGCUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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