Results 21 - 25 of 25 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 1586 | 3' | -52.2 | NC_001347.2 | + | 144672 | 0.67 | 0.988496 |
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Target: 5'- gGUGCgcaACUGCGaCGAACacgaggcucucgacuUGGUGACGcGCg -3' miRNA: 3'- -UACG---UGACGCaGUUUG---------------ACCACUGC-UG- -5' |
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| 1586 | 3' | -52.2 | NC_001347.2 | + | 203015 | 0.67 | 0.982689 |
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Target: 5'- -gGCugUGUgccGUUAAAUaccuugGGUGACGACa -3' miRNA: 3'- uaCGugACG---CAGUUUGa-----CCACUGCUG- -5' |
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| 1586 | 3' | -52.2 | NC_001347.2 | + | 195320 | 0.67 | 0.987904 |
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Target: 5'- -gGCACggggGCGUCGugaaggaggaGACU-GUGAUGACu -3' miRNA: 3'- uaCGUGa---CGCAGU----------UUGAcCACUGCUG- -5' |
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| 1586 | 3' | -52.2 | NC_001347.2 | + | 117865 | 0.67 | 0.986323 |
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Target: 5'- -gGCgACcGCGUCAGAC-GG-GGCGACc -3' miRNA: 3'- uaCG-UGaCGCAGUUUGaCCaCUGCUG- -5' |
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| 1586 | 3' | -52.2 | NC_001347.2 | + | 96652 | 0.73 | 0.81563 |
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Target: 5'- cUGCACcGCGUCAcGCUGGccgaaggcacGACGGCg -3' miRNA: 3'- uACGUGaCGCAGUuUGACCa---------CUGCUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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