Results 61 - 66 of 66 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 15862 | 5' | -57.5 | NC_004065.1 | + | 107420 | 0.66 | 0.926656 |
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Target: 5'- gACugGGaCCUCGUGCUGCccuGGAucgugcccauGGCg -3' miRNA: 3'- -UGugCUaGGGGUACGACGcu-CCU----------CCG- -5' |
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| 15862 | 5' | -57.5 | NC_004065.1 | + | 62107 | 0.66 | 0.926656 |
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Target: 5'- -gGCGGUCgUUAcGCUcGCG-GGAGGCg -3' miRNA: 3'- ugUGCUAGgGGUaCGA-CGCuCCUCCG- -5' |
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| 15862 | 5' | -57.5 | NC_004065.1 | + | 67983 | 0.66 | 0.926656 |
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Target: 5'- cGCAgGAauggaCCAUGUacUGCGGGGAGGa -3' miRNA: 3'- -UGUgCUagg--GGUACG--ACGCUCCUCCg -5' |
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| 15862 | 5' | -57.5 | NC_004065.1 | + | 130172 | 0.66 | 0.926656 |
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Target: 5'- cCGCGA-CCCCGgcgccaucUGgUGCGAcGGGGCc -3' miRNA: 3'- uGUGCUaGGGGU--------ACgACGCUcCUCCG- -5' |
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| 15862 | 5' | -57.5 | NC_004065.1 | + | 71025 | 0.66 | 0.926656 |
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Target: 5'- aGCugGAggCCaagagaGUGgucgGCGGGGAGGCa -3' miRNA: 3'- -UGugCUagGGg-----UACga--CGCUCCUCCG- -5' |
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| 15862 | 5' | -57.5 | NC_004065.1 | + | 200365 | 0.66 | 0.926656 |
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Target: 5'- -gGCG-UCCUCuucGCcgGCGAGGAGGg -3' miRNA: 3'- ugUGCuAGGGGua-CGa-CGCUCCUCCg -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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