Results 61 - 66 of 66 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 15862 | 5' | -57.5 | NC_004065.1 | + | 193694 | 0.67 | 0.871425 |
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Target: 5'- -gAUGcgCCgUCAcgGCcGCGAGGAGGCa -3' miRNA: 3'- ugUGCuaGG-GGUa-CGaCGCUCCUCCG- -5' |
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| 15862 | 5' | -57.5 | NC_004065.1 | + | 194345 | 0.71 | 0.67876 |
|
Target: 5'- cGCGCGAUCUCCugGCUcGCGAGcGAcguGGCg -3' miRNA: 3'- -UGUGCUAGGGGuaCGA-CGCUC-CU---CCG- -5' |
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| 15862 | 5' | -57.5 | NC_004065.1 | + | 200365 | 0.66 | 0.926656 |
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Target: 5'- -gGCG-UCCUCuucGCcgGCGAGGAGGg -3' miRNA: 3'- ugUGCuAGGGGua-CGa-CGCUCCUCCg -5' |
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| 15862 | 5' | -57.5 | NC_004065.1 | + | 208193 | 0.67 | 0.891122 |
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Target: 5'- cCGCGGUaucCCCCggGCggGCGAGGAuucaagaGGUg -3' miRNA: 3'- uGUGCUA---GGGGuaCGa-CGCUCCU-------CCG- -5' |
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| 15862 | 5' | -57.5 | NC_004065.1 | + | 215812 | 0.69 | 0.764569 |
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Target: 5'- gGCACGcgUUUCGUGCgauauUGUGAcGGAGGCg -3' miRNA: 3'- -UGUGCuaGGGGUACG-----ACGCU-CCUCCG- -5' |
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| 15862 | 5' | -57.5 | NC_004065.1 | + | 229568 | 0.71 | 0.698288 |
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Target: 5'- cCAgGGUCCCCGgacguuacGCcGCGAGGGcGGCu -3' miRNA: 3'- uGUgCUAGGGGUa-------CGaCGCUCCU-CCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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