miRNA: 3'- aCGUaGGGaCCCGGCGGCuAGGcCUg -5'
miRNA: 3'- aCGUa--GGGAC-CCGGCGGCua--GGCCUG- -5'
miRNA: 3'- aCGUAGGGAC-CCGG---CGGC--UAGG-CCUG- -5'
miRNA: 3'- -ACGuAGGGaC-CCGGCGGCua-GGC-CUG- -5'
Results 41 - 44 of 44 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
|
Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 15866 | 5' | -62.1 | NC_004065.1 | + | 131768 | 0.7 | 0.526308 |
|
Target: 5'- cGCG-CCCaGGGCCGCCG-UCCcGAg -3' miRNA: 3'- aCGUaGGGaCCCGGCGGCuAGGcCUg -5' |
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| 15866 | 5' | -62.1 | NC_004065.1 | + | 151513 | 0.69 | 0.535578 |
|
Target: 5'- aGCGgugUUCUGaGGCCGCUGccggCCGGGCg -3' miRNA: 3'- aCGUa--GGGAC-CCGGCGGCua--GGCCUG- -5' |
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| 15866 | 5' | -62.1 | NC_004065.1 | + | 78487 | 0.69 | 0.563708 |
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Target: 5'- gGUGUCCUUGcGGCCcuuGCCGccGUCCaGGGCg -3' miRNA: 3'- aCGUAGGGAC-CCGG---CGGC--UAGG-CCUG- -5' |
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| 15866 | 5' | -62.1 | NC_004065.1 | + | 203603 | 0.66 | 0.761907 |
|
Target: 5'- gUGC-UCUCgGaGGCCGCCGccgCCGuGGCg -3' miRNA: 3'- -ACGuAGGGaC-CCGGCGGCua-GGC-CUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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