Results 41 - 47 of 47 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
|
Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 15867 | 3' | -55.2 | NC_004065.1 | + | 217163 | 0.72 | 0.753411 |
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Target: 5'- cGCUCGuucucguguguGCU-CUCGUCGAGGUGGuuGg -3' miRNA: 3'- -CGAGCu----------CGAuGGGUAGCUUCACCggC- -5' |
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| 15867 | 3' | -55.2 | NC_004065.1 | + | 176137 | 0.71 | 0.832514 |
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Target: 5'- aCUCGAaCUGuuuCCCGUCGAcgccaacggAGUGGCCa -3' miRNA: 3'- cGAGCUcGAU---GGGUAGCU---------UCACCGGc -5' |
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| 15867 | 3' | -55.2 | NC_004065.1 | + | 173942 | 0.68 | 0.915732 |
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Target: 5'- uGCUgCGAGCagaaccucauCgCCAUCGggGUGGUCc -3' miRNA: 3'- -CGA-GCUCGau--------G-GGUAGCuuCACCGGc -5' |
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| 15867 | 3' | -55.2 | NC_004065.1 | + | 143649 | 0.68 | 0.92127 |
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Target: 5'- gGC-CGAGacCUACCC--CGAcGUGGCCGa -3' miRNA: 3'- -CGaGCUC--GAUGGGuaGCUuCACCGGC- -5' |
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| 15867 | 3' | -55.2 | NC_004065.1 | + | 195336 | 0.68 | 0.931665 |
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Target: 5'- uGCUCcuGGGCggcGCCCGcgucUUGAAgGUGGCCa -3' miRNA: 3'- -CGAG--CUCGa--UGGGU----AGCUU-CACCGGc -5' |
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| 15867 | 3' | -55.2 | NC_004065.1 | + | 202199 | 0.68 | 0.931665 |
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Target: 5'- gGC-CGAGC-ACCCGcaggcacaUCGGAG-GGCUGg -3' miRNA: 3'- -CGaGCUCGaUGGGU--------AGCUUCaCCGGC- -5' |
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| 15867 | 3' | -55.2 | NC_004065.1 | + | 127464 | 0.66 | 0.975641 |
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Target: 5'- -gUCGAuCUcCCCGggGAGGUGGUCGg -3' miRNA: 3'- cgAGCUcGAuGGGUagCUUCACCGGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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