Results 21 - 27 of 27 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 15886 | 5' | -53.9 | NC_004065.1 | + | 203163 | 0.69 | 0.929919 |
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Target: 5'- aCCCGAGGaccGuCUCUCgACUucCAGCUGUg -3' miRNA: 3'- -GGGCUCU---CuGAGAG-UGAu-GUCGACGa -5' |
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| 15886 | 5' | -53.9 | NC_004065.1 | + | 158809 | 0.69 | 0.918647 |
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Target: 5'- gCCCGAucuccgacuucagGAGGCUCgu-CUucaGCAGCUGCg -3' miRNA: 3'- -GGGCU-------------CUCUGAGaguGA---UGUCGACGa -5' |
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| 15886 | 5' | -53.9 | NC_004065.1 | + | 130332 | 0.7 | 0.895003 |
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Target: 5'- uCCCGGGAG-CUCUCGCUcuuugucaccggAC-GCUGUc -3' miRNA: 3'- -GGGCUCUCuGAGAGUGA------------UGuCGACGa -5' |
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| 15886 | 5' | -53.9 | NC_004065.1 | + | 205218 | 0.7 | 0.888385 |
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Target: 5'- aCCCGAaaGGGCUCUCGCUcugcauCGGCaUGCg -3' miRNA: 3'- -GGGCUc-UCUGAGAGUGAu-----GUCG-ACGa -5' |
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| 15886 | 5' | -53.9 | NC_004065.1 | + | 109344 | 0.71 | 0.87449 |
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Target: 5'- gCUGGGAGACauguacuucgUCUCGCgcgACAcGCUGCg -3' miRNA: 3'- gGGCUCUCUG----------AGAGUGa--UGU-CGACGa -5' |
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| 15886 | 5' | -53.9 | NC_004065.1 | + | 74777 | 0.71 | 0.859748 |
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Target: 5'- uUCCGcAGGGAgUCUCGCgGCAGC-GCUc -3' miRNA: 3'- -GGGC-UCUCUgAGAGUGaUGUCGaCGA- -5' |
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| 15886 | 5' | -53.9 | NC_004065.1 | + | 44200 | 1.11 | 0.005303 |
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Target: 5'- aCCCGAGAGACUCUCACUACAGCUGCUa -3' miRNA: 3'- -GGGCUCUCUGAGAGUGAUGUCGACGA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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