Results 1 - 14 of 14 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
159 | 5' | -57.3 | AC_000007.1 | + | 15168 | 0.66 | 0.466795 |
Target: 5'- -gCCAcacGGGCGgaggAGaaGCGCGCUGAGGc -3' miRNA: 3'- gaGGUu--CCCGU----UCgaCGCGCGGUUCC- -5' |
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159 | 5' | -57.3 | AC_000007.1 | + | 6636 | 0.66 | 0.466795 |
Target: 5'- --gCGGGcGGCAAGC-GCGCGCUcguauGGGu -3' miRNA: 3'- gagGUUC-CCGUUCGaCGCGCGGu----UCC- -5' |
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159 | 5' | -57.3 | AC_000007.1 | + | 4308 | 0.66 | 0.446459 |
Target: 5'- uUUCAGuaGCAAGCUGauUGCCAGGGg -3' miRNA: 3'- gAGGUUccCGUUCGACgcGCGGUUCC- -5' |
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159 | 5' | -57.3 | AC_000007.1 | + | 10767 | 0.66 | 0.436478 |
Target: 5'- uUCCAGGcGCGgcGGCUGCuGCGCUAGc- -3' miRNA: 3'- gAGGUUCcCGU--UCGACG-CGCGGUUcc -5' |
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159 | 5' | -57.3 | AC_000007.1 | + | 12538 | 0.67 | 0.416909 |
Target: 5'- -gCguGGGGUuuGC-GCGCGCCGGGa -3' miRNA: 3'- gaGguUCCCGuuCGaCGCGCGGUUCc -5' |
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159 | 5' | -57.3 | AC_000007.1 | + | 5330 | 0.68 | 0.335021 |
Target: 5'- ---aAGGGGUgcgcuccGGGCUGCGCgcugGCCAGGGu -3' miRNA: 3'- gaggUUCCCG-------UUCGACGCG----CGGUUCC- -5' |
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159 | 5' | -57.3 | AC_000007.1 | + | 26239 | 0.69 | 0.322705 |
Target: 5'- --gCAAGGGCccgcggcugcugauaGGGCUGCGgCGgCGGGGg -3' miRNA: 3'- gagGUUCCCG---------------UUCGACGC-GCgGUUCC- -5' |
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159 | 5' | -57.3 | AC_000007.1 | + | 15705 | 0.69 | 0.303708 |
Target: 5'- --gUggGGGCuGGCggGCGCGCCAAa- -3' miRNA: 3'- gagGuuCCCGuUCGa-CGCGCGGUUcc -5' |
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159 | 5' | -57.3 | AC_000007.1 | + | 25301 | 0.69 | 0.29605 |
Target: 5'- -cUCAAGGGCGAG--GCGCGCCGc-- -3' miRNA: 3'- gaGGUUCCCGUUCgaCGCGCGGUucc -5' |
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159 | 5' | -57.3 | AC_000007.1 | + | 23546 | 0.69 | 0.281187 |
Target: 5'- ---gAAGGGCAuggaGGCUGCGCGCgCGuugauGGa -3' miRNA: 3'- gaggUUCCCGU----UCGACGCGCG-GUu----CC- -5' |
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159 | 5' | -57.3 | AC_000007.1 | + | 5566 | 0.7 | 0.24663 |
Target: 5'- uUCCGGGGaGUAGGCaucCGCGCCGcAGGc -3' miRNA: 3'- gAGGUUCC-CGUUCGac-GCGCGGU-UCC- -5' |
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159 | 5' | -57.3 | AC_000007.1 | + | 8646 | 0.71 | 0.221581 |
Target: 5'- -gCCGcgcgcGGGCAGgagcugguGCUGCGCGCgGAGGu -3' miRNA: 3'- gaGGUu----CCCGUU--------CGACGCGCGgUUCC- -5' |
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159 | 5' | -57.3 | AC_000007.1 | + | 25099 | 0.74 | 0.134277 |
Target: 5'- cCUCCAAGucGGCAGGCUcGCGCGUcuCAAGc -3' miRNA: 3'- -GAGGUUC--CCGUUCGA-CGCGCG--GUUCc -5' |
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159 | 5' | -57.3 | AC_000007.1 | + | 5536 | 1.11 | 0.000222 |
Target: 5'- cCUCCAAGGGCAAGCUGCGCGCCAAGGg -3' miRNA: 3'- -GAGGUUCCCGUUCGACGCGCGGUUCC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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