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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 1590 | 3' | -51.4 | NC_001347.2 | + | 209498 | 0.66 | 0.99807 |
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Target: 5'- uCCUGUGACCUccuauucaguucuccUUGUcccgcuGGAUaagugaccUGCGGCUCu -3' miRNA: 3'- -GGAUACUGGA---------------AGCA------CCUA--------GCGCUGAG- -5' |
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| 1590 | 3' | -51.4 | NC_001347.2 | + | 93725 | 0.66 | 0.997116 |
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Target: 5'- aCUcAUGACCgcguaUCuUGGAagGCGGCUCc -3' miRNA: 3'- gGA-UACUGGa----AGcACCUagCGCUGAG- -5' |
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| 1590 | 3' | -51.4 | NC_001347.2 | + | 193233 | 0.67 | 0.996617 |
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Target: 5'- aCUUGgccGACCUgugCGUGcGA-CGCGACUa -3' miRNA: 3'- -GGAUa--CUGGAa--GCAC-CUaGCGCUGAg -5' |
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| 1590 | 3' | -51.4 | NC_001347.2 | + | 21635 | 0.67 | 0.994677 |
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Target: 5'- gCCgcaGGCCUUUGUcGG-CGCGGCUCu -3' miRNA: 3'- -GGauaCUGGAAGCAcCUaGCGCUGAG- -5' |
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| 1590 | 3' | -51.4 | NC_001347.2 | + | 174778 | 0.67 | 0.994677 |
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Target: 5'- ----aGACCggcggcgaCGUGGGUCGCGAgUUCa -3' miRNA: 3'- ggauaCUGGaa------GCACCUAGCGCU-GAG- -5' |
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| 1590 | 3' | -51.4 | NC_001347.2 | + | 9474 | 0.7 | 0.965353 |
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Target: 5'- ----cGACCUUUGUGG--CGCGACUg -3' miRNA: 3'- ggauaCUGGAAGCACCuaGCGCUGAg -5' |
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| 1590 | 3' | -51.4 | NC_001347.2 | + | 200453 | 0.74 | 0.847474 |
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Target: 5'- gCC--UGGCCUUCGUGGGUCGC--UUCg -3' miRNA: 3'- -GGauACUGGAAGCACCUAGCGcuGAG- -5' |
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| 1590 | 3' | -51.4 | NC_001347.2 | + | 178658 | 1.13 | 0.008028 |
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Target: 5'- uCCUAUGACCUUCGUGGAUCGCGACUCg -3' miRNA: 3'- -GGAUACUGGAAGCACCUAGCGCUGAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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