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miRNA: 3'- -UAG-CGGAGCGGU------AGUGuauUugGGGa -5'
miRNA: 3'- -UAGCGGA---------GCGGUAg-UGUAuUUGGGGA- -5'
miRNA: 3'- -UAGCGGaGCGGUAgUGUauuuGGGGa -5'
miRNA: 3'- uagCGG-AGCGGUAGUGUa---UUUGGgGA- -5'
Results 41 - 44 of 44 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position |
R_P_ratio#
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P value |
| Predicted miRNA align pattern | |||||||
| 15905 | 5' | -53.9 | NC_004065.1 | + | 127860 | 0.66 | 0.973375 |
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Target: 5'- cGUCuGCCUCGCCAgguaccUCACc--GugCCCg -3' miRNA: 3'- -UAG-CGGAGCGGU------AGUGuauUugGGGa -5' |
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| 15905 | 5' | -53.9 | NC_004065.1 | + | 63718 | 0.66 | 0.975966 |
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Target: 5'- gAUCGCCUgguggucggCGCCGUagACAU-AGCCCUUg -3' miRNA: 3'- -UAGCGGA---------GCGGUAg-UGUAuUUGGGGA- -5' |
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| 15905 | 5' | -53.9 | NC_004065.1 | + | 91791 | 0.66 | 0.970589 |
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Target: 5'- cGUCGUC-CGCCAUgGCGaccuCCCCg -3' miRNA: 3'- -UAGCGGaGCGGUAgUGUauuuGGGGa -5' |
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| 15905 | 5' | -53.9 | NC_004065.1 | + | 87269 | 0.66 | 0.980586 |
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Target: 5'- --gGCCgUCGUCGUCGCGggagAGACCgCUa -3' miRNA: 3'- uagCGG-AGCGGUAGUGUa---UUUGGgGA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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