miRNA: 3'- uAGCGGA-------GCGGUAGugUaUUUGGGGA- -5'
miRNA: 3'- uaGCGGAgCGGuaGUGUAUUUG-GGGa -5'
miRNA: 3'- -UAGc---GGAGCGGUAGUGU----AUUUGG-GGa -5'
miRNA: 3'- uagCGGAG--CGGUAGUGUA----------UUUGGGGa -5'
Results 41 - 44 of 44 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
|
R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 15905 | 5' | -53.9 | NC_004065.1 | + | 196151 | 0.68 | 0.944643 |
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Target: 5'- cUCGCCUgggacucCGCCAUCugG-GGGCUCCUc -3' miRNA: 3'- uAGCGGA-------GCGGUAGugUaUUUGGGGA- -5' |
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| 15905 | 5' | -53.9 | NC_004065.1 | + | 196542 | 0.7 | 0.874806 |
|
Target: 5'- -aCGCCU-GCCggCGCGUAGGCgCCCa -3' miRNA: 3'- uaGCGGAgCGGuaGUGUAUUUG-GGGa -5' |
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| 15905 | 5' | -53.9 | NC_004065.1 | + | 210963 | 0.67 | 0.967599 |
|
Target: 5'- gGUCuccuCCUCGUCGUCACAgaugUGGGCCaCCg -3' miRNA: 3'- -UAGc---GGAGCGGUAGUGU----AUUUGG-GGa -5' |
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| 15905 | 5' | -53.9 | NC_004065.1 | + | 221294 | 0.66 | 0.973375 |
|
Target: 5'- --gGCCUCcgGUCGUCACGUuuucgauaacAGGCCCCc -3' miRNA: 3'- uagCGGAG--CGGUAGUGUA----------UUUGGGGa -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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