miRNA: 3'- uaGCGG--AGCGGuAGU-GUAUUUG-GGGA- -5'
miRNA: 3'- -UAGCGG-AGcGGUAGUGUauUUGGGGa -5'
miRNA: 3'- uagCGGAGcGGUAGUGuaUUUGGGGa -5'
miRNA: 3'- -UAGCGGAgCGGU---AGuGUAUU------UGGGGA- -5'
Results 41 - 44 of 44 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
|
R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 15905 | 5' | -53.9 | NC_004065.1 | + | 29484 | 0.69 | 0.895532 |
|
Target: 5'- -cCGCCcgUCGCCcUCGcCAUGGACgCCCUg -3' miRNA: 3'- uaGCGG--AGCGGuAGU-GUAUUUG-GGGA- -5' |
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| 15905 | 5' | -53.9 | NC_004065.1 | + | 20642 | 0.67 | 0.953494 |
|
Target: 5'- uAUCGCCgUCuCCGUCGCA--GGCCUCg -3' miRNA: 3'- -UAGCGG-AGcGGUAGUGUauUUGGGGa -5' |
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| 15905 | 5' | -53.9 | NC_004065.1 | + | 16707 | 0.66 | 0.978367 |
|
Target: 5'- --aGCCUC-CCAUCAUccAAACCUCg -3' miRNA: 3'- uagCGGAGcGGUAGUGuaUUUGGGGa -5' |
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| 15905 | 5' | -53.9 | NC_004065.1 | + | 5307 | 0.67 | 0.957352 |
|
Target: 5'- cGUUGCCUgGCCAacaUC-CGUGGgucgacACCCCUg -3' miRNA: 3'- -UAGCGGAgCGGU---AGuGUAUU------UGGGGA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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