miRNA display CGI


Results 1 - 11 of 11 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
1591 3' -58.3 NC_001347.2 + 143475 0.66 0.906462
Target:  5'- gGGCGCUGAuGGCCUaCUCGcgccagccGGACcggGCg -3'
miRNA:   3'- -CUGCGGCUcCCGGA-GAGU--------CCUGaa-CG- -5'
1591 3' -58.3 NC_001347.2 + 8342 0.66 0.900431
Target:  5'- aGCGCCGAGGGUaggugcgUUUCGGcGCcgUGCa -3'
miRNA:   3'- cUGCGGCUCCCGg------AGAGUCcUGa-ACG- -5'
1591 3' -58.3 NC_001347.2 + 181752 0.66 0.900431
Target:  5'- uGCGCCGAcGGCgUUUCAaGGugU-GCg -3'
miRNA:   3'- cUGCGGCUcCCGgAGAGU-CCugAaCG- -5'
1591 3' -58.3 NC_001347.2 + 189150 0.66 0.900431
Target:  5'- cGCGCCGcGGugacacaaacaGCgUCUCGGGACgccGCu -3'
miRNA:   3'- cUGCGGCuCC-----------CGgAGAGUCCUGaa-CG- -5'
1591 3' -58.3 NC_001347.2 + 184396 0.66 0.881062
Target:  5'- gGGCGuuGcGGGGCUUCUU-GGACUcGUg -3'
miRNA:   3'- -CUGCggC-UCCCGGAGAGuCCUGAaCG- -5'
1591 3' -58.3 NC_001347.2 + 193253 0.66 0.874192
Target:  5'- cGACGCgacuaUGAGGGCCUg-CGGcGuuACUUGCg -3'
miRNA:   3'- -CUGCG-----GCUCCCGGAgaGUC-C--UGAACG- -5'
1591 3' -58.3 NC_001347.2 + 8236 0.66 0.872091
Target:  5'- -cCGCCGuGGGCggugauaaccaguugCUCAGGACacUGCa -3'
miRNA:   3'- cuGCGGCuCCCGga-------------GAGUCCUGa-ACG- -5'
1591 3' -58.3 NC_001347.2 + 150157 0.69 0.741198
Target:  5'- aAUGCCGAGGGCCgcgCcCAGGuag-GCc -3'
miRNA:   3'- cUGCGGCUCCCGGa--GaGUCCugaaCG- -5'
1591 3' -58.3 NC_001347.2 + 930 0.69 0.731806
Target:  5'- cGGgGCCgGAGGGCCU-UCGGGGgUUGg -3'
miRNA:   3'- -CUgCGG-CUCCCGGAgAGUCCUgAACg -5'
1591 3' -58.3 NC_001347.2 + 94083 0.7 0.711832
Target:  5'- -cUGCCGucGGGCCagaccagUCUCAGGugUaGCg -3'
miRNA:   3'- cuGCGGCu-CCCGG-------AGAGUCCugAaCG- -5'
1591 3' -58.3 NC_001347.2 + 177752 1.12 0.001739
Target:  5'- cGACGCCGAGGGCCUCUCAGGACUUGCg -3'
miRNA:   3'- -CUGCGGCUCCCGGAGAGUCCUGAACG- -5' 
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.