Results 61 - 63 of 63 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position![]() |
R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
1592 | 3' | -56.7 | NC_001347.2 | + | 1732 | 0.72 | 0.71275 |
Target: 5'- gCCGGCGgugggC-CGGCACgaCGGUgGCGCu -3' miRNA: 3'- gGGCCGCaa---GaGCCGUG--GCCAaUGCG- -5' |
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1592 | 3' | -56.7 | NC_001347.2 | + | 1495 | 0.74 | 0.596815 |
Target: 5'- aCCUGGCG--CUgGGCGCCGGguucCGCg -3' miRNA: 3'- -GGGCCGCaaGAgCCGUGGCCaau-GCG- -5' |
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1592 | 3' | -56.7 | NC_001347.2 | + | 1210 | 0.68 | 0.916261 |
Target: 5'- gCgCGGCGUUUUUGGCcaacaGCaCGGgccGCGCc -3' miRNA: 3'- -GgGCCGCAAGAGCCG-----UG-GCCaa-UGCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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