Results 21 - 27 of 27 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 1592 | 5' | -49.4 | NC_001347.2 | + | 32460 | 0.69 | 0.994912 |
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Target: 5'- -uGCGUAAUUGACGGUGGaAACa--- -3' miRNA: 3'- acCGCAUUAACUGCCACUcUUGagcc -5' |
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| 1592 | 5' | -49.4 | NC_001347.2 | + | 163414 | 0.69 | 0.994912 |
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Target: 5'- -cGCGUAAUUGugGGUGuc-GCUUGa -3' miRNA: 3'- acCGCAUUAACugCCACucuUGAGCc -5' |
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| 1592 | 5' | -49.4 | NC_001347.2 | + | 142144 | 0.7 | 0.993214 |
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Target: 5'- cUGGCGcug--GACGccGAGAACUUGGa -3' miRNA: 3'- -ACCGCauuaaCUGCcaCUCUUGAGCC- -5' |
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| 1592 | 5' | -49.4 | NC_001347.2 | + | 148604 | 0.71 | 0.986959 |
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Target: 5'- cGGCGUGAccGGCGGUGcGGucgCGGg -3' miRNA: 3'- aCCGCAUUaaCUGCCACuCUugaGCC- -5' |
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| 1592 | 5' | -49.4 | NC_001347.2 | + | 40648 | 0.71 | 0.983477 |
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Target: 5'- gGGUGUGcggGACGGUGuuGGGGC-CGGg -3' miRNA: 3'- aCCGCAUuaaCUGCCAC--UCUUGaGCC- -5' |
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| 1592 | 5' | -49.4 | NC_001347.2 | + | 108029 | 0.73 | 0.954936 |
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Target: 5'- gGGUGUGG-UGACGGUGGGGcUUCGc -3' miRNA: 3'- aCCGCAUUaACUGCCACUCUuGAGCc -5' |
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| 1592 | 5' | -49.4 | NC_001347.2 | + | 177575 | 1.13 | 0.012992 |
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Target: 5'- aUGGCGUAAUUGACGGUGAGAACUCGGa -3' miRNA: 3'- -ACCGCAUUAACUGCCACUCUUGAGCC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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