Results 21 - 27 of 27 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
1592 | 5' | -49.4 | NC_001347.2 | + | 171772 | 0.67 | 0.999501 |
Target: 5'- cGGCGUccgAAUgGugGGUaGAGAGCgcgcUGGa -3' miRNA: 3'- aCCGCA---UUAaCugCCA-CUCUUGa---GCC- -5' |
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1592 | 5' | -49.4 | NC_001347.2 | + | 177575 | 1.13 | 0.012992 |
Target: 5'- aUGGCGUAAUUGACGGUGAGAACUCGGa -3' miRNA: 3'- -ACCGCAUUAACUGCCACUCUUGAGCC- -5' |
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1592 | 5' | -49.4 | NC_001347.2 | + | 198086 | 0.67 | 0.999501 |
Target: 5'- cGGCGgucgcGACGGUGAcGGucccGCUCGu -3' miRNA: 3'- aCCGCauuaaCUGCCACU-CU----UGAGCc -5' |
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1592 | 5' | -49.4 | NC_001347.2 | + | 199238 | 0.69 | 0.995622 |
Target: 5'- cGuGCGUGAUUauccGACGcGUGAGAucgcGCUCGc -3' miRNA: 3'- aC-CGCAUUAA----CUGC-CACUCU----UGAGCc -5' |
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1592 | 5' | -49.4 | NC_001347.2 | + | 209979 | 0.68 | 0.998067 |
Target: 5'- uUGGCGUAGUUGACGGaGucccgcaccGC-CGGg -3' miRNA: 3'- -ACCGCAUUAACUGCCaCucu------UGaGCC- -5' |
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1592 | 5' | -49.4 | NC_001347.2 | + | 225551 | 0.68 | 0.998883 |
Target: 5'- cGGCGcucg-GACGGaugggGAGAACggCGGu -3' miRNA: 3'- aCCGCauuaaCUGCCa----CUCUUGa-GCC- -5' |
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1592 | 5' | -49.4 | NC_001347.2 | + | 229691 | 0.67 | 0.999079 |
Target: 5'- gUGGCGggGUgugucGGCGGUGugcgcGGcCUCGGg -3' miRNA: 3'- -ACCGCauUAa----CUGCCACu----CUuGAGCC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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