Results 21 - 27 of 27 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 1592 | 5' | -49.4 | NC_001347.2 | + | 63066 | 0.69 | 0.997282 |
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Target: 5'- gUGGCGgagGAcugccGGCgGGUGuGGGCUCGGc -3' miRNA: 3'- -ACCGCa--UUaa---CUG-CCACuCUUGAGCC- -5' |
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| 1592 | 5' | -49.4 | NC_001347.2 | + | 55522 | 0.68 | 0.998067 |
|
Target: 5'- aGGCGUGu---ACGGUGGGAGgUCu- -3' miRNA: 3'- aCCGCAUuaacUGCCACUCUUgAGcc -5' |
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| 1592 | 5' | -49.4 | NC_001347.2 | + | 40648 | 0.71 | 0.983477 |
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Target: 5'- gGGUGUGcggGACGGUGuuGGGGC-CGGg -3' miRNA: 3'- aCCGCAUuaaCUGCCAC--UCUUGaGCC- -5' |
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| 1592 | 5' | -49.4 | NC_001347.2 | + | 40101 | 0.66 | 0.999841 |
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Target: 5'- gGGCGaGGgacGACGGcccUGGGGACcgCGGg -3' miRNA: 3'- aCCGCaUUaa-CUGCC---ACUCUUGa-GCC- -5' |
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| 1592 | 5' | -49.4 | NC_001347.2 | + | 32460 | 0.69 | 0.994912 |
|
Target: 5'- -uGCGUAAUUGACGGUGGaAACa--- -3' miRNA: 3'- acCGCAUUAACUGCCACUcUUGagcc -5' |
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| 1592 | 5' | -49.4 | NC_001347.2 | + | 5545 | 0.67 | 0.999149 |
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Target: 5'- aGGCGUGcucguaucccggcaaGUUGcGCGGUGGGGGCg--- -3' miRNA: 3'- aCCGCAU---------------UAAC-UGCCACUCUUGagcc -5' |
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| 1592 | 5' | -49.4 | NC_001347.2 | + | 212 | 0.67 | 0.999079 |
|
Target: 5'- gUGGCGggGUgugucGGCGGUGugcgcGGcCUCGGg -3' miRNA: 3'- -ACCGCauUAa----CUGCCACu----CUuGAGCC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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