miRNA: 3'- -UGa---AGCC-CGUCCUAuGGUGCCaCUGC- -5'
miRNA: 3'- uGAAGC-CCGuCCUAUGGUGCCACUGC- -5'
miRNA: 3'- -UGAAGCCCGUCCUAUGGUGCCACUGC- -5'
Results 41 - 43 of 43 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 15925 | 3' | -55.3 | NC_004065.1 | + | 35601 | 0.76 | 0.549159 |
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Target: 5'- gGCggugUCGGuGCGGGAU-CCACGGcGGCGg -3' miRNA: 3'- -UGa---AGCC-CGUCCUAuGGUGCCaCUGC- -5' |
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| 15925 | 3' | -55.3 | NC_004065.1 | + | 105656 | 0.85 | 0.176981 |
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Target: 5'- cUUUCGcGGC-GGAUGCCGCGGUGACGa -3' miRNA: 3'- uGAAGC-CCGuCCUAUGGUGCCACUGC- -5' |
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| 15925 | 3' | -55.3 | NC_004065.1 | + | 5619 | 1.08 | 0.006571 |
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Target: 5'- gACUUCGGGCAGGAUACCACGGUGACGc -3' miRNA: 3'- -UGAAGCCCGUCCUAUGGUGCCACUGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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