miRNA: 3'- -UGUGGCA-CCuGCACaUGCGCAGGCcu -5'
miRNA: 3'- uGUGGcACCUGC---ACAUGCGC----AGGCcu -5'
miRNA: 3'- -UGUGGCACCUgcaCAUGCGCaGGCCu -5'
miRNA: 3'- -UGU-GGCaCCUGCACAugcgCAGGCCU- -5'
miRNA: 3'- uguggCACCugCA-CAUGCgCAGgCCU- -5'
Results 21 - 25 of 25 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 15926 | 3' | -57 | NC_004065.1 | + | 119628 | 0.66 | 0.940295 |
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Target: 5'- gACGCCGUcGGcCGUG-GCGUGUUCGa- -3' miRNA: 3'- -UGUGGCA-CCuGCACaUGCGCAGGCcu -5' |
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| 15926 | 3' | -57 | NC_004065.1 | + | 29497 | 0.66 | 0.944656 |
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Target: 5'- uCGCCaUGGACGcccUGUGCGCGgcgaUCCGa- -3' miRNA: 3'- uGUGGcACCUGC---ACAUGCGC----AGGCcu -5' |
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| 15926 | 3' | -57 | NC_004065.1 | + | 6672 | 0.66 | 0.944656 |
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Target: 5'- cGCAUCGUGGGaagGUACGUGgCUGGu -3' miRNA: 3'- -UGUGGCACCUgcaCAUGCGCaGGCCu -5' |
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| 15926 | 3' | -57 | NC_004065.1 | + | 141163 | 0.66 | 0.948804 |
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Target: 5'- gGCAcCCG-GGACGUGUuucaGUUCGGAc -3' miRNA: 3'- -UGU-GGCaCCUGCACAugcgCAGGCCU- -5' |
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| 15926 | 3' | -57 | NC_004065.1 | + | 185469 | 0.66 | 0.95274 |
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Target: 5'- ----gGUGGugGUgGUGCGgGUCgGGAg -3' miRNA: 3'- uguggCACCugCA-CAUGCgCAGgCCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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