Results 21 - 25 of 25 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position![]() |
R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
15926 | 3' | -57 | NC_004065.1 | + | 176491 | 0.7 | 0.80927 |
Target: 5'- gGCACCG-GGACG-GUGgGUGUCCugucucGGAc -3' miRNA: 3'- -UGUGGCaCCUGCaCAUgCGCAGG------CCU- -5' |
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15926 | 3' | -57 | NC_004065.1 | + | 185469 | 0.66 | 0.95274 |
Target: 5'- ----gGUGGugGUgGUGCGgGUCgGGAg -3' miRNA: 3'- uguggCACCugCA-CAUGCgCAGgCCU- -5' |
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15926 | 3' | -57 | NC_004065.1 | + | 186394 | 0.68 | 0.884784 |
Target: 5'- gGCugCGuUGGugGUu--CGUGUCUGGAa -3' miRNA: 3'- -UGugGC-ACCugCAcauGCGCAGGCCU- -5' |
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15926 | 3' | -57 | NC_004065.1 | + | 208862 | 0.67 | 0.930923 |
Target: 5'- -aGCCGUucgagacggagaGGACG-GUGCGCGgugaCGGAc -3' miRNA: 3'- ugUGGCA------------CCUGCaCAUGCGCag--GCCU- -5' |
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15926 | 3' | -57 | NC_004065.1 | + | 223976 | 0.67 | 0.930923 |
Target: 5'- uACACCGUc--UGUGagcaACGUGUCCGGAc -3' miRNA: 3'- -UGUGGCAccuGCACa---UGCGCAGGCCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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