Results 21 - 25 of 25 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. |
strand![]() |
Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
15926 | 3' | -57 | NC_004065.1 | + | 71541 | 0.67 | 0.925911 |
Target: 5'- uAUAUCGcgGGACGg--ACGUGUUCGGGg -3' miRNA: 3'- -UGUGGCa-CCUGCacaUGCGCAGGCCU- -5' |
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15926 | 3' | -57 | NC_004065.1 | + | 97813 | 0.67 | 0.930923 |
Target: 5'- uCACCGgcaGGuuGUGUACGCGg-UGGAa -3' miRNA: 3'- uGUGGCa--CCugCACAUGCGCagGCCU- -5' |
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15926 | 3' | -57 | NC_004065.1 | + | 208862 | 0.67 | 0.930923 |
Target: 5'- -aGCCGUucgagacggagaGGACG-GUGCGCGgugaCGGAc -3' miRNA: 3'- ugUGGCA------------CCUGCaCAUGCGCag--GCCU- -5' |
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15926 | 3' | -57 | NC_004065.1 | + | 6672 | 0.66 | 0.944656 |
Target: 5'- cGCAUCGUGGGaagGUACGUGgCUGGu -3' miRNA: 3'- -UGUGGCACCUgcaCAUGCGCaGGCCu -5' |
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15926 | 3' | -57 | NC_004065.1 | + | 1829 | 0.76 | 0.453327 |
Target: 5'- --cCCGUGGugacggugauCGUGUugGUGUCCGGAa -3' miRNA: 3'- uguGGCACCu---------GCACAugCGCAGGCCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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