Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 1593 | 5' | -57 | NC_001347.2 | + | 199933 | 0.66 | 0.930015 |
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Target: 5'- --cCUCAUCCGCCgGCGuGUCCUcGUUGc -3' miRNA: 3'- cccGAGUGGGUGGaCGU-CAGGA-CAAC- -5' |
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| 1593 | 5' | -57 | NC_001347.2 | + | 203316 | 0.66 | 0.930015 |
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Target: 5'- cGGGCUCGCCgGCCcuUGCGccgCUUGg-- -3' miRNA: 3'- -CCCGAGUGGgUGG--ACGUca-GGACaac -5' |
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| 1593 | 5' | -57 | NC_001347.2 | + | 51442 | 0.67 | 0.908089 |
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Target: 5'- uGGGCUCgcguuuugauaACCUACCUGguGUCg----- -3' miRNA: 3'- -CCCGAG-----------UGGGUGGACguCAGgacaac -5' |
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| 1593 | 5' | -57 | NC_001347.2 | + | 78077 | 0.67 | 0.9063 |
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Target: 5'- cGGGCUCugCCGCCaccaaaagaucUGCcAGuugcucaauaccuuUCCUGUg- -3' miRNA: 3'- -CCCGAGugGGUGG-----------ACG-UC--------------AGGACAac -5' |
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| 1593 | 5' | -57 | NC_001347.2 | + | 23905 | 0.67 | 0.898318 |
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Target: 5'- -uGCUCACCUugugucucuaugagaACCUGguGUaCCUGUa- -3' miRNA: 3'- ccCGAGUGGG---------------UGGACguCA-GGACAac -5' |
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| 1593 | 5' | -57 | NC_001347.2 | + | 200222 | 0.7 | 0.760269 |
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Target: 5'- cGGGCUCGCCCACUgacucgGCGcGUCacaUGa-- -3' miRNA: 3'- -CCCGAGUGGGUGGa-----CGU-CAGg--ACaac -5' |
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| 1593 | 5' | -57 | NC_001347.2 | + | 13513 | 0.71 | 0.683523 |
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Target: 5'- gGGuGCUCACCCGCCggGCGGcCCgGg-- -3' miRNA: 3'- -CC-CGAGUGGGUGGa-CGUCaGGaCaac -5' |
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| 1593 | 5' | -57 | NC_001347.2 | + | 28490 | 0.72 | 0.66377 |
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Target: 5'- uGGCUCuucaUCGCCgUGCAGUCCUGg-- -3' miRNA: 3'- cCCGAGug--GGUGG-ACGUCAGGACaac -5' |
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| 1593 | 5' | -57 | NC_001347.2 | + | 176671 | 1.09 | 0.003346 |
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Target: 5'- uGGGCUCACCCACCUGCAGUCCUGUUGc -3' miRNA: 3'- -CCCGAGUGGGUGGACGUCAGGACAAC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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