Results 21 - 26 of 26 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 15933 | 3' | -55.4 | NC_004065.1 | + | 143513 | 0.69 | 0.874181 |
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Target: 5'- gGGAuGCUGUG-AGC-AGCG-UCCUCGAc -3' miRNA: 3'- -UCU-CGGCACuUCGcUCGCaAGGAGCU- -5' |
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| 15933 | 3' | -55.4 | NC_004065.1 | + | 4409 | 0.7 | 0.862585 |
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Target: 5'- cGGAGCgGUGGccacGGCGGGCcugacgcugacccucGggCCUCGAg -3' miRNA: 3'- -UCUCGgCACU----UCGCUCG---------------CaaGGAGCU- -5' |
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| 15933 | 3' | -55.4 | NC_004065.1 | + | 42698 | 0.7 | 0.859605 |
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Target: 5'- cGGAGCUGcaGAggucGGCGAGCGcUCCUCc- -3' miRNA: 3'- -UCUCGGCa-CU----UCGCUCGCaAGGAGcu -5' |
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| 15933 | 3' | -55.4 | NC_004065.1 | + | 24971 | 0.71 | 0.793986 |
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Target: 5'- -cGGUCGUG-GGUGGGCGUcCCUCGGc -3' miRNA: 3'- ucUCGGCACuUCGCUCGCAaGGAGCU- -5' |
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| 15933 | 3' | -55.4 | NC_004065.1 | + | 229408 | 0.8 | 0.326528 |
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Target: 5'- aAGAGCgGUGAAGCGAcUGUUgCCUCGAa -3' miRNA: 3'- -UCUCGgCACUUCGCUcGCAA-GGAGCU- -5' |
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| 15933 | 3' | -55.4 | NC_004065.1 | + | 573 | 1.09 | 0.004971 |
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Target: 5'- gAGAGCCGUGAAGCGAGCGUUCCUCGAg -3' miRNA: 3'- -UCUCGGCACUUCGCUCGCAAGGAGCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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