Results 21 - 26 of 26 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position![]() |
R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
15933 | 3' | -55.4 | NC_004065.1 | + | 169176 | 0.68 | 0.918491 |
Target: 5'- cGGGCCGgucucGAuGUGAGUGUucgccgccgUCCUCGGu -3' miRNA: 3'- uCUCGGCa----CUuCGCUCGCA---------AGGAGCU- -5' |
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15933 | 3' | -55.4 | NC_004065.1 | + | 194813 | 0.66 | 0.96273 |
Target: 5'- --cGCUGU--AGCGAGCGgUCCUcCGAg -3' miRNA: 3'- ucuCGGCAcuUCGCUCGCaAGGA-GCU- -5' |
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15933 | 3' | -55.4 | NC_004065.1 | + | 196624 | 0.67 | 0.93412 |
Target: 5'- cGGAugcGCCGg--GGCGAGCG--CCUCGAg -3' miRNA: 3'- -UCU---CGGCacuUCGCUCGCaaGGAGCU- -5' |
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15933 | 3' | -55.4 | NC_004065.1 | + | 211146 | 0.66 | 0.965644 |
Target: 5'- -uGGCCGUucgggggGGAGCGGGCGUgCCggGAu -3' miRNA: 3'- ucUCGGCA-------CUUCGCUCGCAaGGagCU- -5' |
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15933 | 3' | -55.4 | NC_004065.1 | + | 216251 | 0.66 | 0.965957 |
Target: 5'- uGAGCCGacgacacggUGAucuCGGGC-UUCCUCGAa -3' miRNA: 3'- uCUCGGC---------ACUuc-GCUCGcAAGGAGCU- -5' |
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15933 | 3' | -55.4 | NC_004065.1 | + | 229408 | 0.8 | 0.326528 |
Target: 5'- aAGAGCgGUGAAGCGAcUGUUgCCUCGAa -3' miRNA: 3'- -UCUCGgCACUUCGCUcGCAA-GGAGCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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