Results 21 - 28 of 28 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 15935 | 3' | -54.2 | NC_004065.1 | + | 97647 | 0.67 | 0.957352 |
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Target: 5'- cGGAGAa-GGGCgACGCGacGGCCUGCcCg -3' miRNA: 3'- uCUUCUcaCCCG-UGCGU--UCGGAUGuG- -5' |
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| 15935 | 3' | -54.2 | NC_004065.1 | + | 211401 | 0.67 | 0.957352 |
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Target: 5'- uGGcGGAGgcgGGGgaaACGUggGCCUGgGCg -3' miRNA: 3'- -UCuUCUCa--CCCg--UGCGuuCGGAUgUG- -5' |
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| 15935 | 3' | -54.2 | NC_004065.1 | + | 116575 | 0.67 | 0.962378 |
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Target: 5'- uGAuauuGAG-GGGCACgGCGGGCCccccggccgccgcucUGCGCa -3' miRNA: 3'- uCUu---CUCaCCCGUG-CGUUCGG---------------AUGUG- -5' |
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| 15935 | 3' | -54.2 | NC_004065.1 | + | 50326 | 0.66 | 0.967599 |
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Target: 5'- -aGAGAGUGguGGCACGCGuGGCCacCGCc -3' miRNA: 3'- ucUUCUCAC--CCGUGCGU-UCGGauGUG- -5' |
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| 15935 | 3' | -54.2 | NC_004065.1 | + | 149915 | 0.66 | 0.970589 |
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Target: 5'- uGGAuGAG-GGGCGCGC-GGCUcgGCAg -3' miRNA: 3'- -UCUuCUCaCCCGUGCGuUCGGa-UGUg -5' |
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| 15935 | 3' | -54.2 | NC_004065.1 | + | 154335 | 0.66 | 0.970589 |
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Target: 5'- cAGGAG-GUGGGgGCGCGgccaagaaucAGCCcGCGg -3' miRNA: 3'- -UCUUCuCACCCgUGCGU----------UCGGaUGUg -5' |
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| 15935 | 3' | -54.2 | NC_004065.1 | + | 87602 | 0.66 | 0.970876 |
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Target: 5'- uGAGGAGccGGCGCGCcggcaggcgaaagauGGCCcGCGCg -3' miRNA: 3'- uCUUCUCacCCGUGCGu--------------UCGGaUGUG- -5' |
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| 15935 | 3' | -54.2 | NC_004065.1 | + | 116859 | 0.66 | 0.975463 |
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Target: 5'- uGGAGGAGUugaagaucaucaGGCugGCGuGCCUGCu- -3' miRNA: 3'- -UCUUCUCAc-----------CCGugCGUuCGGAUGug -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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