Results 61 - 63 of 63 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position |
R_P_ratio![]() |
P value |
Predicted miRNA align pattern | |||||||
1594 | 3' | -52.5 | NC_001347.2 | + | 197723 | 0.66 | 0.996202 |
Target: 5'- cUCGGGCCUgCGacGCGACGCCGaCGg-- -3' miRNA: 3'- -GGUCUGGAgGU--UGUUGUGGCaGCagc -5' |
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1594 | 3' | -52.5 | NC_001347.2 | + | 189897 | 0.66 | 0.996744 |
Target: 5'- gCCGGGCCgccUCGACcACGCCGaCGUa- -3' miRNA: 3'- -GGUCUGGa--GGUUGuUGUGGCaGCAgc -5' |
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1594 | 3' | -52.5 | NC_001347.2 | + | 134389 | 0.66 | 0.99722 |
Target: 5'- cCCu--CCUCCAagAUGGCGCCGcucggccagacUCGUCGa -3' miRNA: 3'- -GGucuGGAGGU--UGUUGUGGC-----------AGCAGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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