Results 61 - 68 of 68 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 1595 | 5' | -59.1 | NC_001347.2 | + | 28003 | 0.68 | 0.76805 |
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Target: 5'- gUCGcCGCCGUCACcaCCGCCGAUc- -3' miRNA: 3'- uGGCaGCGGCAGUGacGGUGGCUGua -5' |
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| 1595 | 5' | -59.1 | NC_001347.2 | + | 192124 | 0.68 | 0.784992 |
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Target: 5'- cGCCGccacCGCCGUCGUUGCCGguccccguuuccuCCGGCGUc -3' miRNA: 3'- -UGGCa---GCGGCAGUGACGGU-------------GGCUGUA- -5' |
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| 1595 | 5' | -59.1 | NC_001347.2 | + | 88221 | 0.68 | 0.785872 |
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Target: 5'- cGCCGcucaCGCUguccgagggGUCGCUGCCGCCGGu-- -3' miRNA: 3'- -UGGCa---GCGG---------CAGUGACGGUGGCUgua -5' |
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| 1595 | 5' | -59.1 | NC_001347.2 | + | 463 | 0.68 | 0.785872 |
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Target: 5'- cGCCGUCGCCGUC-CUcCgCACC-ACAc -3' miRNA: 3'- -UGGCAGCGGCAGuGAcG-GUGGcUGUa -5' |
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| 1595 | 5' | -59.1 | NC_001347.2 | + | 159441 | 0.67 | 0.819935 |
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Target: 5'- uCCGUCGUCGUCuGCggccgcgGCCGCuCGAUg- -3' miRNA: 3'- uGGCAGCGGCAG-UGa------CGGUG-GCUGua -5' |
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| 1595 | 5' | -59.1 | NC_001347.2 | + | 113456 | 0.67 | 0.819935 |
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Target: 5'- uGCaCGUaGcCCGUCACggucgGCCGCCGugAg -3' miRNA: 3'- -UG-GCAgC-GGCAGUGa----CGGUGGCugUa -5' |
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| 1595 | 5' | -59.1 | NC_001347.2 | + | 101689 | 0.67 | 0.819935 |
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Target: 5'- cACgCGUCugGCCGaaGCgGCCACCGAUAg -3' miRNA: 3'- -UG-GCAG--CGGCagUGaCGGUGGCUGUa -5' |
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| 1595 | 5' | -59.1 | NC_001347.2 | + | 103241 | 0.65 | 0.879479 |
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Target: 5'- uGCCGacgagcCGCUGUUugUggacgauuaccguGCCACCGACGa -3' miRNA: 3'- -UGGCa-----GCGGCAGugA-------------CGGUGGCUGUa -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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