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miRNA: 3'- -GUCGCGUUGUCGa-------G-GGGGGAGCGCac -5'
miRNA: 3'- gucgCGUUGUCGAGGGGGGAGCGCAc -5'
miRNA: 3'- -GUCGCGUUGUCGAGGGGGGAGCGCAC- -5'
Results 41 - 43 of 43 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position |
R_P_ratio#
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P value |
| Predicted miRNA align pattern | |||||||
| 15974 | 5' | -60.6 | NC_004065.1 | + | 163254 | 0.76 | 0.257358 |
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Target: 5'- uCGGCGCAGCGGCggcgccggCgCCUCCUCGCGa- -3' miRNA: 3'- -GUCGCGUUGUCGa-------G-GGGGGAGCGCac -5' |
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| 15974 | 5' | -60.6 | NC_004065.1 | + | 162683 | 0.79 | 0.177062 |
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Target: 5'- ---gGCAGCGGCUUCCCUCUCGCGUa -3' miRNA: 3'- gucgCGUUGUCGAGGGGGGAGCGCAc -5' |
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| 15974 | 5' | -60.6 | NC_004065.1 | + | 35399 | 1.08 | 0.001902 |
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Target: 5'- gCAGCGCAACAGCUCCCCCCUCGCGUGg -3' miRNA: 3'- -GUCGCGUUGUCGAGGGGGGAGCGCAC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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