miRNA: 3'- -CAGCAGCuUGUGCaaCUGGAGGCacu -5'
miRNA: 3'- -CAGCAGCUU---GUGCa--------AC-UGGAGGCACU- -5'
miRNA: 3'- -CAGCAGCUUGUGCAACUGGAGGCACU- -5'
Results 21 - 23 of 23 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 15982 | 5' | -51.9 | NC_004065.1 | + | 164185 | 0.73 | 0.879122 |
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Target: 5'- uGUCGUCGuGCGCGcgGAUCUCCGc-- -3' miRNA: 3'- -CAGCAGCuUGUGCaaCUGGAGGCacu -5' |
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| 15982 | 5' | -51.9 | NC_004065.1 | + | 138531 | 0.74 | 0.833237 |
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Target: 5'- cGUCGUCGAGgauCACGgcgacgucgUGcACCUCUGUGAc -3' miRNA: 3'- -CAGCAGCUU---GUGCa--------AC-UGGAGGCACU- -5' |
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| 15982 | 5' | -51.9 | NC_004065.1 | + | 41008 | 1.1 | 0.010923 |
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Target: 5'- cGUCGUCGAACACGUUGACCUCCGUGAg -3' miRNA: 3'- -CAGCAGCUUGUGCAACUGGAGGCACU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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