Results 41 - 44 of 44 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
15986 | 3' | -54.8 | NC_004065.1 | + | 36181 | 0.66 | 0.96699 |
Target: 5'- -cGaAGCGAcgGCAGCAggaauGGUGGCGGUu-- -3' miRNA: 3'- gaC-UCGCU--UGUCGU-----CCACCGCUAcua -5' |
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15986 | 3' | -54.8 | NC_004065.1 | + | 54197 | 0.66 | 0.96699 |
Target: 5'- -aGAGCGAuGCGGUAGGgacaucGGCGAa--- -3' miRNA: 3'- gaCUCGCU-UGUCGUCCa-----CCGCUacua -5' |
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15986 | 3' | -54.8 | NC_004065.1 | + | 127219 | 0.66 | 0.97001 |
Target: 5'- -gGAGCGGcgGCcgcgaggaucgGGCGcGGUGGCGggGAUg -3' miRNA: 3'- gaCUCGCU--UG-----------UCGU-CCACCGCuaCUA- -5' |
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15986 | 3' | -54.8 | NC_004065.1 | + | 116362 | 0.66 | 0.97001 |
Target: 5'- -aGAGCGAGagGGCGGGgaacccGGCGcUGAg -3' miRNA: 3'- gaCUCGCUUg-UCGUCCa-----CCGCuACUa -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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