miRNA: 3'- -GACUCGCU-----------UGUCGuCCACCGCUACUA- -5'
miRNA: 3'- gaCUCGCUUGU-CGUCCa-CCG-CUACUa -5'
miRNA: 3'- gACUCGCUUGUCGucCACCGCuACUa -5'
miRNA: 3'- -GACUCGCUUGUCGUCCACCGCUACUA- -5'
Results 41 - 44 of 44 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 15986 | 3' | -54.8 | NC_004065.1 | + | 225159 | 0.73 | 0.685364 |
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Target: 5'- cCUGGGUGAccgagccgaggGCGGC-GGUGGUGGUGGUu -3' miRNA: 3'- -GACUCGCU-----------UGUCGuCCACCGCUACUA- -5' |
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| 15986 | 3' | -54.8 | NC_004065.1 | + | 23570 | 0.73 | 0.665105 |
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Target: 5'- -cGGGCGGGCAgGCAGGcgGGCuGGUGAa -3' miRNA: 3'- gaCUCGCUUGU-CGUCCa-CCG-CUACUa -5' |
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| 15986 | 3' | -54.8 | NC_004065.1 | + | 104001 | 0.76 | 0.533629 |
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Target: 5'- gUGGGCGAGCGGCucGUGGCGcUGGa -3' miRNA: 3'- gACUCGCUUGUCGucCACCGCuACUa -5' |
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| 15986 | 3' | -54.8 | NC_004065.1 | + | 44019 | 1.05 | 0.008263 |
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Target: 5'- cCUGAGCGAACAGCAGGUGGCGAUGAUc -3' miRNA: 3'- -GACUCGCUUGUCGUCCACCGCUACUA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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