Results 21 - 23 of 23 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value![]() |
Predicted miRNA align pattern | |||||||
1600 | 5' | -56.3 | NC_001347.2 | + | 154889 | 0.77 | 0.410012 |
Target: 5'- uCUGCaucuaGGUCGCAGGCGGcCGAAGCg- -3' miRNA: 3'- -GAUGc----CCAGCGUCCGUCuGCUUCGac -5' |
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1600 | 5' | -56.3 | NC_001347.2 | + | 13947 | 0.81 | 0.246381 |
Target: 5'- -gGCGGG-CGCGGGguGGCGAAGCg- -3' miRNA: 3'- gaUGCCCaGCGUCCguCUGCUUCGac -5' |
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1600 | 5' | -56.3 | NC_001347.2 | + | 164234 | 1.07 | 0.005107 |
Target: 5'- uCUACGGGUCGCAGGCAGACGAAGCUGu -3' miRNA: 3'- -GAUGCCCAGCGUCCGUCUGCUUCGAC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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