Results 21 - 23 of 23 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position![]() |
R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
1600 | 5' | -56.3 | NC_001347.2 | + | 191259 | 0.68 | 0.884705 |
Target: 5'- -cACGGcGUaaUGCGGGCAGGCGugcGGCa- -3' miRNA: 3'- gaUGCC-CA--GCGUCCGUCUGCu--UCGac -5' |
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1600 | 5' | -56.3 | NC_001347.2 | + | 221196 | 0.67 | 0.915738 |
Target: 5'- gUACGGuccUCGCAGGUGGcACGGuAGUUGg -3' miRNA: 3'- gAUGCCc--AGCGUCCGUC-UGCU-UCGAC- -5' |
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1600 | 5' | -56.3 | NC_001347.2 | + | 228240 | 0.69 | 0.824324 |
Target: 5'- gUGCGGGUCGUuucGGCG--UGAAGUUGg -3' miRNA: 3'- gAUGCCCAGCGu--CCGUcuGCUUCGAC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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