Results 41 - 45 of 45 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
16002 | 3' | -55.7 | NC_004065.1 | + | 129328 | 0.66 | 0.973284 |
Target: 5'- aGGAccaGGGGGUCauccgaUCCGAG-CGGGAUAa -3' miRNA: 3'- gCCU---CUCCUAGc-----AGGCUCuGUCCUGU- -5' |
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16002 | 3' | -55.7 | NC_004065.1 | + | 164777 | 0.66 | 0.967711 |
Target: 5'- aCGGAGGacGGGUUGgagauugUCGAGACGGG-CGc -3' miRNA: 3'- -GCCUCU--CCUAGCa------GGCUCUGUCCuGU- -5' |
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16002 | 3' | -55.7 | NC_004065.1 | + | 19709 | 0.66 | 0.967711 |
Target: 5'- aCGcGGAGGcgUGcggccaUCCGAGAUGGGGCGc -3' miRNA: 3'- -GCcUCUCCuaGC------AGGCUCUGUCCUGU- -5' |
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16002 | 3' | -55.7 | NC_004065.1 | + | 187201 | 0.66 | 0.967711 |
Target: 5'- cCGGAGGGGGUUcccCCGAuGugAgGGGCAu -3' miRNA: 3'- -GCCUCUCCUAGca-GGCU-CugU-CCUGU- -5' |
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16002 | 3' | -55.7 | NC_004065.1 | + | 145719 | 0.66 | 0.961361 |
Target: 5'- aGGAGAGG-UCGguggagGAGGCGGGAg- -3' miRNA: 3'- gCCUCUCCuAGCagg---CUCUGUCCUgu -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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