Results 21 - 29 of 29 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 1601 | 5' | -61.2 | NC_001347.2 | + | 126075 | 0.66 | 0.788158 |
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Target: 5'- aCCUGGc-CGCGCGCCAGCccaucccccUCUCGGc -3' miRNA: 3'- cGGGCCcaGCGCGUGGUCGc--------AGAGCU- -5' |
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| 1601 | 5' | -61.2 | NC_001347.2 | + | 209052 | 0.66 | 0.799127 |
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Target: 5'- cGCUCGcgguugcGGUCGCGCACCGGCugcagacagggCGAa -3' miRNA: 3'- -CGGGC-------CCAGCGCGUGGUCGcaga-------GCU- -5' |
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| 1601 | 5' | -61.2 | NC_001347.2 | + | 200688 | 0.66 | 0.77958 |
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Target: 5'- cGCCCGcuGUUGCGCGCCuGgaGcCUCGGu -3' miRNA: 3'- -CGGGCc-CAGCGCGUGGuCg-CaGAGCU- -5' |
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| 1601 | 5' | -61.2 | NC_001347.2 | + | 39808 | 0.66 | 0.788158 |
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Target: 5'- uCCCGGG-CGCGCACCcaucuaGGUGgacgCcCGAc -3' miRNA: 3'- cGGGCCCaGCGCGUGG------UCGCa---GaGCU- -5' |
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| 1601 | 5' | -61.2 | NC_001347.2 | + | 86230 | 0.66 | 0.77958 |
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Target: 5'- cGCUCGGccaCGCGCuCCAGgGcCUCGGc -3' miRNA: 3'- -CGGGCCca-GCGCGuGGUCgCaGAGCU- -5' |
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| 1601 | 5' | -61.2 | NC_001347.2 | + | 100903 | 0.67 | 0.770887 |
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Target: 5'- cUCCGGGUCGCGCugCuuuccccgAGC-UC-CGAc -3' miRNA: 3'- cGGGCCCAGCGCGugG--------UCGcAGaGCU- -5' |
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| 1601 | 5' | -61.2 | NC_001347.2 | + | 171923 | 0.67 | 0.762087 |
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Target: 5'- uGCCgCGG--UGCGCGCUAGCGUCgauuugugcaagUCGGg -3' miRNA: 3'- -CGG-GCCcaGCGCGUGGUCGCAG------------AGCU- -5' |
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| 1601 | 5' | -61.2 | NC_001347.2 | + | 215172 | 0.67 | 0.770887 |
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Target: 5'- -gCCGGacCGUGCAauUCAGCGUCUUGAc -3' miRNA: 3'- cgGGCCcaGCGCGU--GGUCGCAGAGCU- -5' |
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| 1601 | 5' | -61.2 | NC_001347.2 | + | 96920 | 0.8 | 0.169229 |
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Target: 5'- aGCCCGGGcCGC-CGCCAGCGUC-CGc -3' miRNA: 3'- -CGGGCCCaGCGcGUGGUCGCAGaGCu -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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