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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 1603 | 3' | -58.1 | NC_001347.2 | + | 177422 | 0.66 | 0.900098 |
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Target: 5'- aUCUGUUaGGUAGcGUGGGC-CUGCUg -3' miRNA: 3'- cAGACAG-CCGUCaCGCCUGuGACGGa -5' |
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| 1603 | 3' | -58.1 | NC_001347.2 | + | 148594 | 0.67 | 0.839099 |
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Target: 5'- aUCUGagcggcggcgugacCGGCGGUGCGGuCGCgggugGCCg -3' miRNA: 3'- cAGACa-------------GCCGUCACGCCuGUGa----CGGa -5' |
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| 1603 | 3' | -58.1 | NC_001347.2 | + | 84620 | 0.67 | 0.81959 |
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Target: 5'- cGUC-GUgGGUGGccucGCGGugGCUGCCg -3' miRNA: 3'- -CAGaCAgCCGUCa---CGCCugUGACGGa -5' |
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| 1603 | 3' | -58.1 | NC_001347.2 | + | 93138 | 0.7 | 0.700702 |
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Target: 5'- aGUCUGuUCGGCGG-GCGGACcgcucucguuccGCgGCCc -3' miRNA: 3'- -CAGAC-AGCCGUCaCGCCUG------------UGaCGGa -5' |
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| 1603 | 3' | -58.1 | NC_001347.2 | + | 67635 | 0.7 | 0.690944 |
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Target: 5'- uGUCcagCGGCAGcgacaUGCGGcCGCUGCCg -3' miRNA: 3'- -CAGacaGCCGUC-----ACGCCuGUGACGGa -5' |
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| 1603 | 3' | -58.1 | NC_001347.2 | + | 229698 | 0.71 | 0.651536 |
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Target: 5'- gGUgUGUCGGCGGUGU--GCGCgGCCUc -3' miRNA: 3'- -CAgACAGCCGUCACGccUGUGaCGGA- -5' |
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| 1603 | 3' | -58.1 | NC_001347.2 | + | 219 | 0.71 | 0.651536 |
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Target: 5'- gGUgUGUCGGCGGUGU--GCGCgGCCUc -3' miRNA: 3'- -CAgACAGCCGUCACGccUGUGaCGGA- -5' |
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| 1603 | 3' | -58.1 | NC_001347.2 | + | 158202 | 1.06 | 0.003866 |
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Target: 5'- gGUCUGUCGGCAGUGCGGACACUGCCUc -3' miRNA: 3'- -CAGACAGCCGUCACGCCUGUGACGGA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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