miRNA: 3'- gGCcUGUCGUCCGCGCA--GUGcAUCUC- -5'
miRNA: 3'- -GGCCUG-UCGuCCGCGCAGUGCau--CUc -5'
miRNA: 3'- gGCCUGUCGUCCGCGCaGUGCa--UCUC- -5'
miRNA: 3'- -GGCCUGUCGUCCGCGCAGUGCAUCUC- -5'
Results 21 - 24 of 24 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 1604 | 3' | -56.7 | NC_001347.2 | + | 39769 | 0.73 | 0.648427 |
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Target: 5'- aCGcGCGGCAGGCGCGUacCAC-UGGAGc -3' miRNA: 3'- gGCcUGUCGUCCGCGCA--GUGcAUCUC- -5' |
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| 1604 | 3' | -56.7 | NC_001347.2 | + | 16273 | 0.73 | 0.618979 |
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Target: 5'- uCUGGACgAGCuGGCGCGUUACGguauGAu -3' miRNA: 3'- -GGCCUG-UCGuCCGCGCAGUGCau--CUc -5' |
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| 1604 | 3' | -56.7 | NC_001347.2 | + | 55513 | 0.76 | 0.485241 |
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Target: 5'- aUGGGCGGUAGGCGUG-UACGgugGGAGg -3' miRNA: 3'- gGCCUGUCGUCCGCGCaGUGCa--UCUC- -5' |
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| 1604 | 3' | -56.7 | NC_001347.2 | + | 158038 | 1.09 | 0.004159 |
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Target: 5'- gCCGGACAGCAGGCGCGUCACGUAGAGc -3' miRNA: 3'- -GGCCUGUCGUCCGCGCAGUGCAUCUC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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