Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 1605 | 5' | -55.4 | NC_001347.2 | + | 139584 | 0.66 | 0.970931 |
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Target: 5'- gCUCUACGUGCcGGCC-G-UAAGCGa- -3' miRNA: 3'- -GAGAUGCAUGaCCGGgCuGUUCGCca -5' |
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| 1605 | 5' | -55.4 | NC_001347.2 | + | 157055 | 0.66 | 0.970931 |
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Target: 5'- uUCUGCugGUGCUGGCUCugcuGugGcGCGGUc -3' miRNA: 3'- gAGAUG--CAUGACCGGG----CugUuCGCCA- -5' |
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| 1605 | 5' | -55.4 | NC_001347.2 | + | 194389 | 0.66 | 0.964941 |
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Target: 5'- gUCUGCGUGaugcCUGGaCCC-ACAAGCGc- -3' miRNA: 3'- gAGAUGCAU----GACC-GGGcUGUUCGCca -5' |
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| 1605 | 5' | -55.4 | NC_001347.2 | + | 31019 | 0.67 | 0.946314 |
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Target: 5'- gCUCUACGUuacgaaACUGaGCuCCcACAGGUGGa -3' miRNA: 3'- -GAGAUGCA------UGAC-CG-GGcUGUUCGCCa -5' |
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| 1605 | 5' | -55.4 | NC_001347.2 | + | 18182 | 0.67 | 0.94148 |
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Target: 5'- gUCUACG-ACUGGgagaCCGACGGGCucuacgaGGUg -3' miRNA: 3'- gAGAUGCaUGACCg---GGCUGUUCG-------CCA- -5' |
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| 1605 | 5' | -55.4 | NC_001347.2 | + | 196257 | 0.67 | 0.93099 |
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Target: 5'- cCUCUACGcggccuuuugcgagUGCggcgacgGGCgCGACAacGGCGGUa -3' miRNA: 3'- -GAGAUGC--------------AUGa------CCGgGCUGU--UCGCCA- -5' |
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| 1605 | 5' | -55.4 | NC_001347.2 | + | 194691 | 0.7 | 0.825215 |
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Target: 5'- cCUUUGCGUACcuGCCCGGCGAGgacaaGGUg -3' miRNA: 3'- -GAGAUGCAUGacCGGGCUGUUCg----CCA- -5' |
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| 1605 | 5' | -55.4 | NC_001347.2 | + | 36570 | 0.71 | 0.79078 |
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Target: 5'- --aUGCGggaACUGGCCUGGC-GGCGGg -3' miRNA: 3'- gagAUGCa--UGACCGGGCUGuUCGCCa -5' |
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| 1605 | 5' | -55.4 | NC_001347.2 | + | 150440 | 0.74 | 0.61661 |
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Target: 5'- uCUCUACcgccGCUGuGCCCGGCGAcGCGGg -3' miRNA: 3'- -GAGAUGca--UGAC-CGGGCUGUU-CGCCa -5' |
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| 1605 | 5' | -55.4 | NC_001347.2 | + | 157893 | 1.08 | 0.006066 |
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Target: 5'- gCUCUACGUACUGGCCCGACAAGCGGUg -3' miRNA: 3'- -GAGAUGCAUGACCGGGCUGUUCGCCA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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