Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 16056 | 3' | -52.9 | NC_004065.1 | + | 60977 | 0.66 | 0.985918 |
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Target: 5'- aUCGcgcGUACGGC-CGcAGCGCCUUGc- -3' miRNA: 3'- aAGCu--CAUGUCGaGC-UCGUGGAACuu -5' |
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| 16056 | 3' | -52.9 | NC_004065.1 | + | 95774 | 0.66 | 0.979381 |
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Target: 5'- gUCGAcGUGCGGCUCGgugacggguuccagGGCGCCa---- -3' miRNA: 3'- aAGCU-CAUGUCGAGC--------------UCGUGGaacuu -5' |
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| 16056 | 3' | -52.9 | NC_004065.1 | + | 153611 | 0.67 | 0.972541 |
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Target: 5'- aUCGGGU-CGGCUCGGGCggcGCCa---- -3' miRNA: 3'- aAGCUCAuGUCGAGCUCG---UGGaacuu -5' |
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| 16056 | 3' | -52.9 | NC_004065.1 | + | 38942 | 0.67 | 0.972541 |
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Target: 5'- gUCGGGUGuCgAGCUCGAGCgACCg---- -3' miRNA: 3'- aAGCUCAU-G-UCGAGCUCG-UGGaacuu -5' |
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| 16056 | 3' | -52.9 | NC_004065.1 | + | 224651 | 0.67 | 0.969633 |
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Target: 5'- aUCGAGgcuagcACAGUUaCGAGCGCCaaGAGc -3' miRNA: 3'- aAGCUCa-----UGUCGA-GCUCGUGGaaCUU- -5' |
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| 16056 | 3' | -52.9 | NC_004065.1 | + | 20055 | 0.67 | 0.969633 |
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Target: 5'- gUCGGGUGCucucGCUCGAGcCGCCc---- -3' miRNA: 3'- aAGCUCAUGu---CGAGCUC-GUGGaacuu -5' |
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| 16056 | 3' | -52.9 | NC_004065.1 | + | 168826 | 0.68 | 0.959605 |
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Target: 5'- gUUUGGGUAU-GCUCG-GCugCUUGGAg -3' miRNA: 3'- -AAGCUCAUGuCGAGCuCGugGAACUU- -5' |
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| 16056 | 3' | -52.9 | NC_004065.1 | + | 148013 | 0.68 | 0.938227 |
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Target: 5'- aUCGAGUguaugaGCGGCaCGGGCGCCUUc-- -3' miRNA: 3'- aAGCUCA------UGUCGaGCUCGUGGAAcuu -5' |
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| 16056 | 3' | -52.9 | NC_004065.1 | + | 45091 | 0.72 | 0.784257 |
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Target: 5'- gUUCGAcGUGCAGCUCGGGguCCUc--- -3' miRNA: 3'- -AAGCU-CAUGUCGAGCUCguGGAacuu -5' |
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| 16056 | 3' | -52.9 | NC_004065.1 | + | 83625 | 1.04 | 0.013663 |
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Target: 5'- cUUCGAGUACAGCUCGAGCACCUUGAAg -3' miRNA: 3'- -AAGCUCAUGUCGAGCUCGUGGAACUU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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