Results 41 - 48 of 48 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 16058 | 3' | -62 | NC_004065.1 | + | 202310 | 0.73 | 0.361786 |
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Target: 5'- aGUCUCUcaGGAUCUGCUcucgguccGCCGGGCGGAu -3' miRNA: 3'- cUAGAGG--CCUGGGCGG--------UGGCCCGUCU- -5' |
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| 16058 | 3' | -62 | NC_004065.1 | + | 203067 | 0.73 | 0.347107 |
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Target: 5'- cGAgCUCCGGAgCCCGCCgcggccGCCGGaGCGGu -3' miRNA: 3'- -CUaGAGGCCU-GGGCGG------UGGCC-CGUCu -5' |
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| 16058 | 3' | -62 | NC_004065.1 | + | 93344 | 0.75 | 0.280207 |
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Target: 5'- -cUCgagCCGG-CCCGCCGCCGGcCAGAc -3' miRNA: 3'- cuAGa--GGCCuGGGCGGUGGCCcGUCU- -5' |
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| 16058 | 3' | -62 | NC_004065.1 | + | 38749 | 0.75 | 0.266347 |
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Target: 5'- cAUCUCCGGcguGCCCGCgaguaccguuuccaCACCGGGCuGAc -3' miRNA: 3'- cUAGAGGCC---UGGGCG--------------GUGGCCCGuCU- -5' |
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| 16058 | 3' | -62 | NC_004065.1 | + | 130621 | 0.75 | 0.26224 |
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Target: 5'- aGAUCUCCaGcaacgcGCCCGCCGCCaGGCAGc -3' miRNA: 3'- -CUAGAGGcC------UGGGCGGUGGcCCGUCu -5' |
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| 16058 | 3' | -62 | NC_004065.1 | + | 197510 | 0.76 | 0.25079 |
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Target: 5'- aGGUCUCCgagGGACCCgGCCGCgCGcGGUAGAa -3' miRNA: 3'- -CUAGAGG---CCUGGG-CGGUG-GC-CCGUCU- -5' |
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| 16058 | 3' | -62 | NC_004065.1 | + | 18128 | 0.79 | 0.165465 |
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Target: 5'- --cCUCCGGGCCCGCCGC-GGGaCGGGa -3' miRNA: 3'- cuaGAGGCCUGGGCGGUGgCCC-GUCU- -5' |
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| 16058 | 3' | -62 | NC_004065.1 | + | 87473 | 1.08 | 0.001682 |
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Target: 5'- aGAUCUCCGGACCCGCCACCGGGCAGAu -3' miRNA: 3'- -CUAGAGGCCUGGGCGGUGGCCCGUCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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