Results 21 - 29 of 29 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 1607 | 3' | -58 | NC_001347.2 | + | 158677 | 0.66 | 0.878644 |
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Target: 5'- cGGGGcGCgCGCugguuaaACAGACCGCcUGCCa- -3' miRNA: 3'- -CCUCuCG-GCG-------UGUCUGGUGcACGGaa -5' |
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| 1607 | 3' | -58 | NC_001347.2 | + | 165544 | 0.66 | 0.879333 |
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Target: 5'- -aAGGGCCGCG-AGAgCGCGUGCa-- -3' miRNA: 3'- ccUCUCGGCGUgUCUgGUGCACGgaa -5' |
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| 1607 | 3' | -58 | NC_001347.2 | + | 987 | 0.66 | 0.879333 |
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Target: 5'- gGGAGcGCCGUcacaGCGGGCaCugGUGCg-- -3' miRNA: 3'- -CCUCuCGGCG----UGUCUG-GugCACGgaa -5' |
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| 1607 | 3' | -58 | NC_001347.2 | + | 152023 | 0.66 | 0.885443 |
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Target: 5'- aGGAGAGCCggcaaagaagaggGCGCGGAaaCCAcCGUcgGCCg- -3' miRNA: 3'- -CCUCUCGG-------------CGUGUCU--GGU-GCA--CGGaa -5' |
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| 1607 | 3' | -58 | NC_001347.2 | + | 126316 | 0.66 | 0.886112 |
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Target: 5'- cGAGGGCCagGUGCugcuGACCAUG-GCCUa -3' miRNA: 3'- cCUCUCGG--CGUGu---CUGGUGCaCGGAa -5' |
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| 1607 | 3' | -58 | NC_001347.2 | + | 154695 | 0.66 | 0.899032 |
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Target: 5'- uGAGcGCCGCACGGGgCGCGUaaCUg -3' miRNA: 3'- cCUCuCGGCGUGUCUgGUGCAcgGAa -5' |
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| 1607 | 3' | -58 | NC_001347.2 | + | 21782 | 0.66 | 0.899032 |
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Target: 5'- gGGAcuuuGGCUGCuGCGGGCCGCG-GCCc- -3' miRNA: 3'- -CCUc---UCGGCG-UGUCUGGUGCaCGGaa -5' |
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| 1607 | 3' | -58 | NC_001347.2 | + | 78392 | 0.66 | 0.899032 |
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Target: 5'- gGGAGGaCC-CGCGGGCCACGUcgggcugcuGCCg- -3' miRNA: 3'- -CCUCUcGGcGUGUCUGGUGCA---------CGGaa -5' |
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| 1607 | 3' | -58 | NC_001347.2 | + | 136025 | 0.66 | 0.905168 |
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Target: 5'- cGGAGcgGGCCGCGCccggugcccGGcCCACG-GCCc- -3' miRNA: 3'- -CCUC--UCGGCGUG---------UCuGGUGCaCGGaa -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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