Results 21 - 29 of 29 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 1607 | 3' | -58 | NC_001347.2 | + | 174721 | 0.71 | 0.629798 |
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Target: 5'- aGGAGGGCCuGCGCA-ACUACGcGCCg- -3' miRNA: 3'- -CCUCUCGG-CGUGUcUGGUGCaCGGaa -5' |
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| 1607 | 3' | -58 | NC_001347.2 | + | 29230 | 0.71 | 0.609966 |
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Target: 5'- uGGAGGGCUaCGCGGAUCGaGUGCCg- -3' miRNA: 3'- -CCUCUCGGcGUGUCUGGUgCACGGaa -5' |
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| 1607 | 3' | -58 | NC_001347.2 | + | 206705 | 0.72 | 0.60007 |
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Target: 5'- uGGGGGCCGCACGGAgCGCGacGUCg- -3' miRNA: 3'- cCUCUCGGCGUGUCUgGUGCa-CGGaa -5' |
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| 1607 | 3' | -58 | NC_001347.2 | + | 105970 | 0.72 | 0.570541 |
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Target: 5'- cGGcGAGCCGCGCAcGcCCGCGcgGCCg- -3' miRNA: 3'- -CCuCUCGGCGUGU-CuGGUGCa-CGGaa -5' |
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| 1607 | 3' | -58 | NC_001347.2 | + | 145650 | 0.73 | 0.522213 |
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Target: 5'- cGGAGGGuCCGCGCgacacgcaagAGACCACGacgcGCCUc -3' miRNA: 3'- -CCUCUC-GGCGUG----------UCUGGUGCa---CGGAa -5' |
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| 1607 | 3' | -58 | NC_001347.2 | + | 18929 | 0.73 | 0.503316 |
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Target: 5'- -uGGGGCCGCACGGACCuAC-UGCCc- -3' miRNA: 3'- ccUCUCGGCGUGUCUGG-UGcACGGaa -5' |
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| 1607 | 3' | -58 | NC_001347.2 | + | 173966 | 0.74 | 0.45747 |
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Target: 5'- ---cGGCUagaGCGCGGGCCGCGUGCCUg -3' miRNA: 3'- ccucUCGG---CGUGUCUGGUGCACGGAa -5' |
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| 1607 | 3' | -58 | NC_001347.2 | + | 76176 | 0.74 | 0.45479 |
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Target: 5'- uGGAGcugggcGGCCGCACgaagcgauccacguAGGCCACGUGCUc- -3' miRNA: 3'- -CCUC------UCGGCGUG--------------UCUGGUGCACGGaa -5' |
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| 1607 | 3' | -58 | NC_001347.2 | + | 156123 | 1.07 | 0.003349 |
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Target: 5'- gGGAGAGCCGCACAGACCACGUGCCUUg -3' miRNA: 3'- -CCUCUCGGCGUGUCUGGUGCACGGAA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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