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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 16077 | 5' | -49.4 | NC_004065.1 | + | 40106 | 0.66 | 0.99943 |
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Target: 5'- uGUUGUUGgcGGCcgcuauauuGGugGUGgCGAUCUc -3' miRNA: 3'- -CAACAACuaCCG---------CUugCAC-GCUAGAa -5' |
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| 16077 | 5' | -49.4 | NC_004065.1 | + | 7167 | 0.68 | 0.997357 |
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Target: 5'- --cGggGAUGGCGGACGcGCuGUCa- -3' miRNA: 3'- caaCaaCUACCGCUUGCaCGcUAGaa -5' |
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| 16077 | 5' | -49.4 | NC_004065.1 | + | 136659 | 0.68 | 0.996317 |
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Target: 5'- ----cUGGUGGCGAGCGacggGCgGGUCUg -3' miRNA: 3'- caacaACUACCGCUUGCa---CG-CUAGAa -5' |
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| 16077 | 5' | -49.4 | NC_004065.1 | + | 58189 | 0.7 | 0.98507 |
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Target: 5'- aGUUGUUGcuguUGGCGAcggGCaUGCGGUCa- -3' miRNA: 3'- -CAACAACu---ACCGCU---UGcACGCUAGaa -5' |
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| 16077 | 5' | -49.4 | NC_004065.1 | + | 209578 | 0.75 | 0.871217 |
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Target: 5'- ----cUGcUGGCGGGCGUGCGGUCa- -3' miRNA: 3'- caacaACuACCGCUUGCACGCUAGaa -5' |
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| 16077 | 5' | -49.4 | NC_004065.1 | + | 225416 | 0.79 | 0.705314 |
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Target: 5'- -aUGUUGGUGGCGAACG-GuCGGUCg- -3' miRNA: 3'- caACAACUACCGCUUGCaC-GCUAGaa -5' |
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| 16077 | 5' | -49.4 | NC_004065.1 | + | 97897 | 1.07 | 0.022247 |
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Target: 5'- cGUUGUUGAUGGCGAACGUGCGAUCUUg -3' miRNA: 3'- -CAACAACUACCGCUUGCACGCUAGAA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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