Results 21 - 27 of 27 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 16084 | 3' | -57.7 | NC_004065.1 | + | 144983 | 0.7 | 0.701856 |
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Target: 5'- aACAGCUgcGGCGUCAgaugGuGGAUCGCg -3' miRNA: 3'- -UGUCGGgaUCGCGGUa---CuCCUAGCGa -5' |
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| 16084 | 3' | -57.7 | NC_004065.1 | + | 114991 | 0.71 | 0.652359 |
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Target: 5'- uCGGCCCUGGCgGCCGcGcGGAUCuGCg -3' miRNA: 3'- uGUCGGGAUCG-CGGUaCuCCUAG-CGa -5' |
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| 16084 | 3' | -57.7 | NC_004065.1 | + | 30115 | 0.72 | 0.60247 |
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Target: 5'- uGCAGCCCcGGCgGCC-UGGGGAggGCg -3' miRNA: 3'- -UGUCGGGaUCG-CGGuACUCCUagCGa -5' |
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| 16084 | 3' | -57.7 | NC_004065.1 | + | 159953 | 0.72 | 0.592527 |
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Target: 5'- -gGGUCC--GCGCCuuUGAGGAUCGCg -3' miRNA: 3'- ugUCGGGauCGCGGu-ACUCCUAGCGa -5' |
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| 16084 | 3' | -57.7 | NC_004065.1 | + | 99841 | 0.72 | 0.592527 |
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Target: 5'- cACGGCCCcgucgcaccagaUGGCGCC---GGGGUCGCg -3' miRNA: 3'- -UGUCGGG------------AUCGCGGuacUCCUAGCGa -5' |
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| 16084 | 3' | -57.7 | NC_004065.1 | + | 198944 | 0.73 | 0.543349 |
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Target: 5'- gGCGGCCaaGGCcgccGCCAUGAGGAagGCg -3' miRNA: 3'- -UGUCGGgaUCG----CGGUACUCCUagCGa -5' |
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| 16084 | 3' | -57.7 | NC_004065.1 | + | 106029 | 1.07 | 0.003433 |
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Target: 5'- uACAGCCCUAGCGCCAUGAGGAUCGCUa -3' miRNA: 3'- -UGUCGGGAUCGCGGUACUCCUAGCGA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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